Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Ege bölgesi akarsularında yakalanan avrupa yılan balıklarında (Anguilla anguilla Linnaeus, 1758) raphidascaris acus bloch, 1779 (Nematoda : Anisakidae) ’un ilk moleküler karakterizasyonu

Şimşek, Emrah ; Çiloğlu, Arif LU ; Aypak, Süleyman ; Yildirim, Alparslan ; Aldemir, Osman Selçuk ; Ünlü, Ahmet Hakan and Pekmezci, Gökmen Zafer (2016) In Kafkas Universitesi Veteriner Fakultesi Dergisi 22(4). p.529-532
Abstract

In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates... (More)

In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
alternative title
First molecular characterization of Raphidascaris acus (Bloch, 1779) (Nematoda: Anisakidae) from european eels (Anguilla anguilla linnaeus, 1758) caught off the aegean region streams, Turkey
publishing date
type
Contribution to journal
publication status
published
subject
keywords
5.8S, Aegean Region streams, Anguilla anguilla, ITS gene regions, Molecular characterization, Raphidascaris acus
in
Kafkas Universitesi Veteriner Fakultesi Dergisi
volume
22
issue
4
pages
4 pages
publisher
Kafkas University
external identifiers
  • scopus:84964260979
ISSN
1300-6045
DOI
10.9775/kvfd.2016.14980
language
Turkish
LU publication?
no
id
3955d38f-4f6b-4771-a423-be96dba02352
date added to LUP
2017-12-04 15:16:28
date last changed
2022-03-02 02:05:15
@article{3955d38f-4f6b-4771-a423-be96dba02352,
  abstract     = {{<p>In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.</p>}},
  author       = {{Şimşek, Emrah and Çiloğlu, Arif and Aypak, Süleyman and Yildirim, Alparslan and Aldemir, Osman Selçuk and Ünlü, Ahmet Hakan and Pekmezci, Gökmen Zafer}},
  issn         = {{1300-6045}},
  keywords     = {{5.8S; Aegean Region streams; Anguilla anguilla; ITS gene regions; Molecular characterization; Raphidascaris acus}},
  language     = {{tur}},
  month        = {{07}},
  number       = {{4}},
  pages        = {{529--532}},
  publisher    = {{Kafkas University}},
  series       = {{Kafkas Universitesi Veteriner Fakultesi Dergisi}},
  title        = {{Ege bölgesi akarsularında yakalanan avrupa yılan  balıklarında (Anguilla anguilla Linnaeus, 1758)  raphidascaris acus bloch, 1779 (Nematoda : Anisakidae) ’un ilk moleküler karakterizasyonu}},
  url          = {{http://dx.doi.org/10.9775/kvfd.2016.14980}},
  doi          = {{10.9775/kvfd.2016.14980}},
  volume       = {{22}},
  year         = {{2016}},
}