Ege bölgesi akarsularında yakalanan avrupa yılan balıklarında (Anguilla anguilla Linnaeus, 1758) raphidascaris acus bloch, 1779 (Nematoda : Anisakidae) ’un ilk moleküler karakterizasyonu
(2016) In Kafkas Universitesi Veteriner Fakultesi Dergisi 22(4). p.529-532- Abstract
In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates... (More)
In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.
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- author
- Şimşek, Emrah ; Çiloğlu, Arif LU ; Aypak, Süleyman ; Yildirim, Alparslan ; Aldemir, Osman Selçuk ; Ünlü, Ahmet Hakan and Pekmezci, Gökmen Zafer
- alternative title
- First molecular characterization of Raphidascaris acus (Bloch, 1779) (Nematoda: Anisakidae) from european eels (Anguilla anguilla linnaeus, 1758) caught off the aegean region streams, Turkey
- publishing date
- 2016-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5.8S, Aegean Region streams, Anguilla anguilla, ITS gene regions, Molecular characterization, Raphidascaris acus
- in
- Kafkas Universitesi Veteriner Fakultesi Dergisi
- volume
- 22
- issue
- 4
- pages
- 4 pages
- publisher
- Kafkas University
- external identifiers
-
- scopus:84964260979
- ISSN
- 1300-6045
- DOI
- 10.9775/kvfd.2016.14980
- language
- Turkish
- LU publication?
- no
- id
- 3955d38f-4f6b-4771-a423-be96dba02352
- date added to LUP
- 2017-12-04 15:16:28
- date last changed
- 2022-03-02 02:05:15
@article{3955d38f-4f6b-4771-a423-be96dba02352, abstract = {{<p>In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.</p>}}, author = {{Şimşek, Emrah and Çiloğlu, Arif and Aypak, Süleyman and Yildirim, Alparslan and Aldemir, Osman Selçuk and Ünlü, Ahmet Hakan and Pekmezci, Gökmen Zafer}}, issn = {{1300-6045}}, keywords = {{5.8S; Aegean Region streams; Anguilla anguilla; ITS gene regions; Molecular characterization; Raphidascaris acus}}, language = {{tur}}, month = {{07}}, number = {{4}}, pages = {{529--532}}, publisher = {{Kafkas University}}, series = {{Kafkas Universitesi Veteriner Fakultesi Dergisi}}, title = {{Ege bölgesi akarsularında yakalanan avrupa yılan balıklarında (Anguilla anguilla Linnaeus, 1758) raphidascaris acus bloch, 1779 (Nematoda : Anisakidae) ’un ilk moleküler karakterizasyonu}}, url = {{http://dx.doi.org/10.9775/kvfd.2016.14980}}, doi = {{10.9775/kvfd.2016.14980}}, volume = {{22}}, year = {{2016}}, }