Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels
(2010) 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 1. p.578-580- Abstract
We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/395ba1dd-1582-43d7-ae1a-dbec8c649ec9
- author
- Westerlund, F. ; Persson, F. LU ; Kristensen, A. and Tegenfeldt, J. O. LU
- organization
- publishing date
- 2010-12-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA, Fluorescence microscopy, Nanofluidics, Single molecule
- host publication
- 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
- volume
- 1
- pages
- 3 pages
- conference name
- 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
- conference location
- Groningen, Netherlands
- conference dates
- 2010-10-03 - 2010-10-07
- external identifiers
-
- scopus:84884307803
- ISBN
- 9781618390622
- language
- English
- LU publication?
- yes
- id
- 395ba1dd-1582-43d7-ae1a-dbec8c649ec9
- date added to LUP
- 2018-10-20 10:15:58
- date last changed
- 2023-09-08 09:27:53
@inproceedings{395ba1dd-1582-43d7-ae1a-dbec8c649ec9, abstract = {{<p>We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO<sub>2</sub> nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.</p>}}, author = {{Westerlund, F. and Persson, F. and Kristensen, A. and Tegenfeldt, J. O.}}, booktitle = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}}, isbn = {{9781618390622}}, keywords = {{DNA; Fluorescence microscopy; Nanofluidics; Single molecule}}, language = {{eng}}, month = {{12}}, pages = {{578--580}}, title = {{Fluorescence enhancement from single DNA molecules confined in SiO <sub>2</sub> nanochannels}}, volume = {{1}}, year = {{2010}}, }