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Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

Westerlund, F. ; Persson, F. LU ; Kristensen, A. and Tegenfeldt, J. O. LU orcid (2010) 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 1. p.578-580
Abstract

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA, Fluorescence microscopy, Nanofluidics, Single molecule
host publication
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
volume
1
pages
3 pages
conference name
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
conference location
Groningen, Netherlands
conference dates
2010-10-03 - 2010-10-07
external identifiers
  • scopus:84884307803
ISBN
9781618390622
language
English
LU publication?
yes
id
395ba1dd-1582-43d7-ae1a-dbec8c649ec9
date added to LUP
2018-10-20 10:15:58
date last changed
2023-09-08 09:27:53
@inproceedings{395ba1dd-1582-43d7-ae1a-dbec8c649ec9,
  abstract     = {{<p>We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO<sub>2</sub> nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.</p>}},
  author       = {{Westerlund, F. and Persson, F. and Kristensen, A. and Tegenfeldt, J. O.}},
  booktitle    = {{14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}},
  isbn         = {{9781618390622}},
  keywords     = {{DNA; Fluorescence microscopy; Nanofluidics; Single molecule}},
  language     = {{eng}},
  month        = {{12}},
  pages        = {{578--580}},
  title        = {{Fluorescence enhancement from single DNA molecules confined in SiO <sub>2</sub> nanochannels}},
  volume       = {{1}},
  year         = {{2010}},
}