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Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Lundwall, Åke LU ; Hellman, U. ; Eggertsen, G. and Sjöquist, J. (1984) In FEBS Letters 169(1). p.57-62
Abstract
The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.
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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
*Cyanogen Bromide, Complement C3/*analysis, High Pressure Liquid, Gel, Chromatography, Amino Acid Sequence, Amino Acids/analysis, Humans, Peptide Fragments/*analysis, Research Support, Non-U.S. Gov't
in
FEBS Letters
volume
169
issue
1
pages
57 - 62
publisher
Wiley-Blackwell
ISSN
1873-3468
language
English
LU publication?
no
additional info
1
id
90cc740a-84d1-4434-9e04-4a140bfa42b7 (old id 3965056)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6714418
date added to LUP
2016-04-04 14:21:53
date last changed
2020-05-20 08:53:15
@article{90cc740a-84d1-4434-9e04-4a140bfa42b7,
  abstract     = {{The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.}},
  author       = {{Lundwall, Åke and Hellman, U. and Eggertsen, G. and Sjöquist, J.}},
  issn         = {{1873-3468}},
  keywords     = {{*Cyanogen Bromide; Complement C3/*analysis; High Pressure Liquid; Gel; Chromatography; Amino Acid Sequence; Amino Acids/analysis; Humans; Peptide Fragments/*analysis; Research Support; Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{57--62}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3}},
  url          = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6714418}},
  volume       = {{169}},
  year         = {{1984}},
}