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Vaginal and urine self-sampling compared to cervical sampling for HPV-testing with the cobas 4800 HPV test

Asciutto, Katrin Christine LU ; Henningsson, Anna J; Borgfeldt, Henrik; Darlin, Lotten LU and Borgfeldt, Christer LU (2017) In Anticancer Research 37(8). p.4183-4187
Abstract

Background/Aim: To compare human papillomavirus (HPV) DNA detection in self-collected vaginal and urine samples with clinician-taken cervical samples in relation to histology. Materials and Methods: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 218 women with the Cobas 4800 HPV test (Roche Molecular Diagnostics). Results: The sensitivity for detection of HPV in the vaginal self-sampling test was 96.4% and in urine was 83.9% relative to detection by clinician-taken cervical sample. The vaginal self-sampling and the clinician-taken HPV tests had the same sensitivity of 92.8% (95% confidence interval=86.3-96.8%) and specificity for detection of high-grade squamous intraepithelial lesion (HSIL) and... (More)

Background/Aim: To compare human papillomavirus (HPV) DNA detection in self-collected vaginal and urine samples with clinician-taken cervical samples in relation to histology. Materials and Methods: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 218 women with the Cobas 4800 HPV test (Roche Molecular Diagnostics). Results: The sensitivity for detection of HPV in the vaginal self-sampling test was 96.4% and in urine was 83.9% relative to detection by clinician-taken cervical sample. The vaginal self-sampling and the clinician-taken HPV tests had the same sensitivity of 92.8% (95% confidence interval=86.3-96.8%) and specificity for detection of high-grade squamous intraepithelial lesion (HSIL) and adenocarcinoma in situ (AIS). Detection in urine samples had a sensitivity of 76.3% (95% confidence interval=67.9-84.2%) for HSIL/AIS. Conclusion: The Cobas 4800 HPV test detects high-grade pre-cancerous cervical lesions in self-collected vaginal samples with the same high sensitivity as in clinician-taken cervical samples.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytology, HPV analysis, Self-collected vaginal smear, Vaginal smear
in
Anticancer Research
volume
37
issue
8
pages
5 pages
publisher
International Institute of Cancer Research
external identifiers
  • scopus:85026256356
  • wos:000412579000025
ISSN
0250-7005
DOI
10.21873/anticanres.11807
language
English
LU publication?
yes
id
39678402-e031-4552-bbfc-cf8bbcd2b578
date added to LUP
2017-08-22 11:14:24
date last changed
2018-01-16 13:23:10
@article{39678402-e031-4552-bbfc-cf8bbcd2b578,
  abstract     = {<p>Background/Aim: To compare human papillomavirus (HPV) DNA detection in self-collected vaginal and urine samples with clinician-taken cervical samples in relation to histology. Materials and Methods: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 218 women with the Cobas 4800 HPV test (Roche Molecular Diagnostics). Results: The sensitivity for detection of HPV in the vaginal self-sampling test was 96.4% and in urine was 83.9% relative to detection by clinician-taken cervical sample. The vaginal self-sampling and the clinician-taken HPV tests had the same sensitivity of 92.8% (95% confidence interval=86.3-96.8%) and specificity for detection of high-grade squamous intraepithelial lesion (HSIL) and adenocarcinoma in situ (AIS). Detection in urine samples had a sensitivity of 76.3% (95% confidence interval=67.9-84.2%) for HSIL/AIS. Conclusion: The Cobas 4800 HPV test detects high-grade pre-cancerous cervical lesions in self-collected vaginal samples with the same high sensitivity as in clinician-taken cervical samples.</p>},
  author       = {Asciutto, Katrin Christine and Henningsson, Anna J and Borgfeldt, Henrik and Darlin, Lotten and Borgfeldt, Christer},
  issn         = {0250-7005},
  keyword      = {Cytology,HPV analysis,Self-collected vaginal smear,Vaginal smear},
  language     = {eng},
  month        = {08},
  number       = {8},
  pages        = {4183--4187},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer Research},
  title        = {Vaginal and urine self-sampling compared to cervical sampling for HPV-testing with the cobas 4800 HPV test},
  url          = {http://dx.doi.org/10.21873/anticanres.11807},
  volume       = {37},
  year         = {2017},
}