Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: The effect of porous structure and ligand coupling chemistry on protein binding
(2006) In Journal of Molecular Recognition 19(4). p.305-312- Abstract
- Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of... (More)
- Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of the fabricated MPAAG revealed two different types of the porous structures: monomodal macroporous structure with large interconnected pores separated by dense non-porous pore walls in case of plain gels or gels produced via copolymerization with AGE. The other type of the MPAAG (gel produced via co-polymerization with functional monomer carrying IDA-functionality) had bimodal pore structure with large interconnected pores separated by the pore walls pierced through with micropores. The effect of different modifications of MPAAG monoliths and of porous structure of the MPAAG (monomodal and bimodal porous structure) on protein binding has been evaluated. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/397364
- author
- Plieva, Fatima LU ; Bober, Beata ; Dainiak, Maria LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bimodal pore size distribution, IDA, monolith, macroporous gel, cryogel
- in
- Journal of Molecular Recognition
- volume
- 19
- issue
- 4
- pages
- 305 - 312
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000239882300009
- scopus:33747312609
- ISSN
- 1099-1352
- DOI
- 10.1002/jmr.775
- language
- English
- LU publication?
- yes
- id
- e29e079c-81e8-4c1d-a62b-ecae8cff8f99 (old id 397364)
- date added to LUP
- 2016-04-01 12:23:10
- date last changed
- 2022-01-27 02:59:51
@article{e29e079c-81e8-4c1d-a62b-ecae8cff8f99,
abstract = {{Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of the fabricated MPAAG revealed two different types of the porous structures: monomodal macroporous structure with large interconnected pores separated by dense non-porous pore walls in case of plain gels or gels produced via copolymerization with AGE. The other type of the MPAAG (gel produced via co-polymerization with functional monomer carrying IDA-functionality) had bimodal pore structure with large interconnected pores separated by the pore walls pierced through with micropores. The effect of different modifications of MPAAG monoliths and of porous structure of the MPAAG (monomodal and bimodal porous structure) on protein binding has been evaluated. Copyright (c) 2006 John Wiley & Sons, Ltd.}},
author = {{Plieva, Fatima and Bober, Beata and Dainiak, Maria and Galaev, Igor and Mattiasson, Bo}},
issn = {{1099-1352}},
keywords = {{bimodal pore size distribution; IDA; monolith; macroporous gel; cryogel}},
language = {{eng}},
number = {{4}},
pages = {{305--312}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Molecular Recognition}},
title = {{Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: The effect of porous structure and ligand coupling chemistry on protein binding}},
url = {{http://dx.doi.org/10.1002/jmr.775}},
doi = {{10.1002/jmr.775}},
volume = {{19}},
year = {{2006}},
}