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Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: The effect of porous structure and ligand coupling chemistry on protein binding

Plieva, Fatima LU ; Bober, Beata ; Dainiak, Maria LU ; Galaev, Igor LU and Mattiasson, Bo LU (2006) In Journal of Molecular Recognition 19(4). p.305-312
Abstract
Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of... (More)
Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of the fabricated MPAAG revealed two different types of the porous structures: monomodal macroporous structure with large interconnected pores separated by dense non-porous pore walls in case of plain gels or gels produced via copolymerization with AGE. The other type of the MPAAG (gel produced via co-polymerization with functional monomer carrying IDA-functionality) had bimodal pore structure with large interconnected pores separated by the pore walls pierced through with micropores. The effect of different modifications of MPAAG monoliths and of porous structure of the MPAAG (monomodal and bimodal porous structure) on protein binding has been evaluated. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bimodal pore size distribution, IDA, monolith, macroporous gel, cryogel
in
Journal of Molecular Recognition
volume
19
issue
4
pages
305 - 312
publisher
John Wiley and Sons
external identifiers
  • wos:000239882300009
  • scopus:33747312609
ISSN
1099-1352
DOI
10.1002/jmr.775
language
English
LU publication?
yes
id
e29e079c-81e8-4c1d-a62b-ecae8cff8f99 (old id 397364)
date added to LUP
2016-04-01 12:23:10
date last changed
2021-06-08 03:17:20
@article{e29e079c-81e8-4c1d-a62b-ecae8cff8f99,
  abstract     = {Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-(N,N-bis(carboxymethyl)amino-3-allylglycerol). Screening for optimized conditions for the production of the MPAAG with required porous properties was performed in a 96-well chromatographic format that allowed parallel production and analysis of the MPAAG prepared from reaction mixtures with different compositions. Scanning electron microscopy of the fabricated MPAAG revealed two different types of the porous structures: monomodal macroporous structure with large interconnected pores separated by dense non-porous pore walls in case of plain gels or gels produced via copolymerization with AGE. The other type of the MPAAG (gel produced via co-polymerization with functional monomer carrying IDA-functionality) had bimodal pore structure with large interconnected pores separated by the pore walls pierced through with micropores. The effect of different modifications of MPAAG monoliths and of porous structure of the MPAAG (monomodal and bimodal porous structure) on protein binding has been evaluated. Copyright (c) 2006 John Wiley & Sons, Ltd.},
  author       = {Plieva, Fatima and Bober, Beata and Dainiak, Maria and Galaev, Igor and Mattiasson, Bo},
  issn         = {1099-1352},
  language     = {eng},
  number       = {4},
  pages        = {305--312},
  publisher    = {John Wiley and Sons},
  series       = {Journal of Molecular Recognition},
  title        = {Macroporous polyacrylamide monolithic gels with immobilized metal affinity ligands: The effect of porous structure and ligand coupling chemistry on protein binding},
  url          = {http://dx.doi.org/10.1002/jmr.775},
  doi          = {10.1002/jmr.775},
  volume       = {19},
  year         = {2006},
}