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Ion-exchange macroporous hydrophilic gel monolith with grafted polymer brushes

Savina, Irina LU ; Galaev, Igor LU and Mattiasson, Bo LU (2006) Bioaffinity 2005 19(4). p.313-321
Abstract
The grafting of functional polymers to the pore surface of macroporous monolithic polyacrylamide cryogels was found to be an efficient and convenient method for the preparation of macroporous polyacrylamide gels, so-called cryogels (pAAm cryogels), with both controlled extent of functional group incorporated and with tailored surface chemistries. Anion-exchange polymer chains of poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA) and poly([2-(methacryloyloxy)ethyl]-trimethylammonium chloride) (pMETA), and cation-exchange polymer chains of polyacrylate have been grafted onto pAAm cryogels using potassium diperiodatocuprate as initiator. It was possible to achieve the ion-exchange capacity up to 0.2-0.5 mmol/ml. The graft polymerization did... (More)
The grafting of functional polymers to the pore surface of macroporous monolithic polyacrylamide cryogels was found to be an efficient and convenient method for the preparation of macroporous polyacrylamide gels, so-called cryogels (pAAm cryogels), with both controlled extent of functional group incorporated and with tailored surface chemistries. Anion-exchange polymer chains of poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA) and poly([2-(methacryloyloxy)ethyl]-trimethylammonium chloride) (pMETA), and cation-exchange polymer chains of polyacrylate have been grafted onto pAAm cryogels using potassium diperiodatocuprate as initiator. It was possible to achieve the ion-exchange capacity up to 0.2-0.5 mmol/ml. The graft polymerization did not alter the macroporous structure of the pAAm cryogel, however the flow rate of solutes through the cryogel matrix decreased with increase in the density of polymer grafted. The sorption of low-molecular-weight (metal ion, dye) and high-molecular-weight (protein) substances on the grafted monolithic pAAm column has been studied. The results indicate that a 'tentacle'-type binding of protein to grafted polymer depended on the architecture of the grafted polymer layer and took place after a certain degree of grafting has been reached. The binding of proteins by tentacle-like polymer chains allowed for increasing the binding capacity for proteins on the grafted pAAm cryogels up to 6-12 mg/ml. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
graft polymerization, protein binding, tentacle chromatography, macroporous polyacrylamide gel
host publication
Journal of Molecular Recognition
volume
19
issue
4
pages
313 - 321
publisher
John Wiley and Sons
conference name
Bioaffinity 2005
conference location
Upsala, Sweden
conference dates
2005-08-14 - 2005-08-18
external identifiers
  • wos:000239882300010
  • scopus:33747292527
ISSN
1099-1352
0952-3499
DOI
10.1002/jmr.774
language
English
LU publication?
yes
id
5e000d1b-58a2-43fb-ac64-ccd656b3e479 (old id 397370)
date added to LUP
2016-04-01 12:26:37
date last changed
2021-06-16 05:13:02
@inproceedings{5e000d1b-58a2-43fb-ac64-ccd656b3e479,
  abstract     = {The grafting of functional polymers to the pore surface of macroporous monolithic polyacrylamide cryogels was found to be an efficient and convenient method for the preparation of macroporous polyacrylamide gels, so-called cryogels (pAAm cryogels), with both controlled extent of functional group incorporated and with tailored surface chemistries. Anion-exchange polymer chains of poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA) and poly([2-(methacryloyloxy)ethyl]-trimethylammonium chloride) (pMETA), and cation-exchange polymer chains of polyacrylate have been grafted onto pAAm cryogels using potassium diperiodatocuprate as initiator. It was possible to achieve the ion-exchange capacity up to 0.2-0.5 mmol/ml. The graft polymerization did not alter the macroporous structure of the pAAm cryogel, however the flow rate of solutes through the cryogel matrix decreased with increase in the density of polymer grafted. The sorption of low-molecular-weight (metal ion, dye) and high-molecular-weight (protein) substances on the grafted monolithic pAAm column has been studied. The results indicate that a 'tentacle'-type binding of protein to grafted polymer depended on the architecture of the grafted polymer layer and took place after a certain degree of grafting has been reached. The binding of proteins by tentacle-like polymer chains allowed for increasing the binding capacity for proteins on the grafted pAAm cryogels up to 6-12 mg/ml. Copyright (c) 2006 John Wiley & Sons, Ltd.},
  author       = {Savina, Irina and Galaev, Igor and Mattiasson, Bo},
  booktitle    = {Journal of Molecular Recognition},
  issn         = {1099-1352},
  language     = {eng},
  number       = {4},
  pages        = {313--321},
  publisher    = {John Wiley and Sons},
  title        = {Ion-exchange macroporous hydrophilic gel monolith with grafted polymer brushes},
  url          = {http://dx.doi.org/10.1002/jmr.774},
  doi          = {10.1002/jmr.774},
  volume       = {19},
  year         = {2006},
}