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Interaction of sugars, polysaccharides and cells with boronate-containing copolymers: from solution to polymer brushes

Ivanov, Alexander LU ; Galaev, Igor LU and Mattiasson, Bo LU (2006) Bioaffinity 2005 19(4). p.322-331
Abstract
Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30... (More)
Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate-sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA-NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous buffered media with pH from 7.5 to 9.2. In turn, the agarose particles and yeast cells were found to adhere to siliceous supports end-grafted with boronate-BCC of N,N-dimethylacrylamide at pH >= 7.5, due to the actions. Quantitative detachment of adhered particles or cells could be attained by addition of 20 mM or 100 mM fructose, respectively, in the pH range from 7.5 to 9.2. Affinity adhesion of micron-size carbohydrate particles to boronate-containing polymer brushes fixed on solid supports was considered as a model system suggesting a new approach to isolation and separation of living cells. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
thermoresponsive polymers, biomimetic, binding constant, boronic acid, cell adhesion, cell, oligosaccharides, chemisorption, detachment, immobilization
host publication
Journal of Molecular Recognition
volume
19
issue
4
pages
322 - 331
publisher
John Wiley and Sons
conference name
Bioaffinity 2005
conference location
Upsala, Sweden
conference dates
2005-08-14 - 2005-08-18
external identifiers
  • wos:000239882300011
  • scopus:33747246960
ISSN
0952-3499
1099-1352
DOI
10.1002/jmr.792
language
English
LU publication?
yes
id
8c499693-a8bf-4d39-8f26-e4352625e37a (old id 397374)
date added to LUP
2016-04-01 12:15:53
date last changed
2021-02-17 06:53:32
@inproceedings{8c499693-a8bf-4d39-8f26-e4352625e37a,
  abstract     = {Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate-sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA-NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous buffered media with pH from 7.5 to 9.2. In turn, the agarose particles and yeast cells were found to adhere to siliceous supports end-grafted with boronate-BCC of N,N-dimethylacrylamide at pH >= 7.5, due to the actions. Quantitative detachment of adhered particles or cells could be attained by addition of 20 mM or 100 mM fructose, respectively, in the pH range from 7.5 to 9.2. Affinity adhesion of micron-size carbohydrate particles to boronate-containing polymer brushes fixed on solid supports was considered as a model system suggesting a new approach to isolation and separation of living cells. Copyright (c) 2006 John Wiley & Sons, Ltd.},
  author       = {Ivanov, Alexander and Galaev, Igor and Mattiasson, Bo},
  booktitle    = {Journal of Molecular Recognition},
  issn         = {0952-3499},
  language     = {eng},
  number       = {4},
  pages        = {322--331},
  publisher    = {John Wiley and Sons},
  title        = {Interaction of sugars, polysaccharides and cells with boronate-containing copolymers: from solution to polymer brushes},
  url          = {http://dx.doi.org/10.1002/jmr.792},
  doi          = {10.1002/jmr.792},
  volume       = {19},
  year         = {2006},
}