Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells
(2006) In Journal of Chromatography A 1123(2). p.145-150- Abstract
- Suitable conditions for separating cells using a chromatographic procedure were evaluated in parallel chromatography on minicolumns. A 96-hole minicolumn plate filled with cryogel monoliths (18.8 mm x 7.1 mm O) with immobilized concanavalin A was used. Chromatographic columns (113 mm x 7.1 mm O) were used for chromatographic resolution of a mixture of Saccharomyces cerevisiae and Escherichia coli cells. Separation of a cell mixture containing equal amounts of cells of both types performed in a column format under the determined optimal conditions, resulted in a quantitative capture of applied S. cerevisiae cells, while E. coli passed through the column. Bound S. cerevisiae cells were released by flow-induced detachment and by compression... (More)
- Suitable conditions for separating cells using a chromatographic procedure were evaluated in parallel chromatography on minicolumns. A 96-hole minicolumn plate filled with cryogel monoliths (18.8 mm x 7.1 mm O) with immobilized concanavalin A was used. Chromatographic columns (113 mm x 7.1 mm O) were used for chromatographic resolution of a mixture of Saccharomyces cerevisiae and Escherichia coli cells. Separation of a cell mixture containing equal amounts of cells of both types performed in a column format under the determined optimal conditions, resulted in a quantitative capture of applied S. cerevisiae cells, while E. coli passed through the column. Bound S. cerevisiae cells were released by flow-induced detachment and by compression of the adsorbent in the presence of 0.3 M methyl U-D-manno-pyranoside. The flowthrough and the eluted fractions were analyzed by plate counting and by registering metabolic activity of S. cerevisiae cells in the eluted fractions after capturing on ConA-cryogel monoliths in a 96-minicolumn plate format. The flowthrough fraction contained E. coli cells with nearly 100% purity, whereas the fraction eluted by compression of the adsorbent contained viable S. cerevisiae cells with 95% purity. Thus, an efficient chromatographic separation of cells was achieved using affinity cryogel column. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/397383
- author
- Dainiak, Maria LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- screening, column format, format, 96-minicolumn plate, elastic, ConA-cryogel monoliths, macroporous, cell chromatography, cell detachment, compression of the adsorbent
- in
- Journal of Chromatography A
- volume
- 1123
- issue
- 2
- pages
- 145 - 150
- publisher
- Elsevier
- external identifiers
-
- wos:000239741400002
- pmid:16846611
- scopus:33746337923
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2006.05.089
- language
- English
- LU publication?
- yes
- id
- e111c585-66f8-43ac-86f7-2fa592aaf2ad (old id 397383)
- date added to LUP
- 2016-04-01 16:59:42
- date last changed
- 2022-03-15 04:22:16
@article{e111c585-66f8-43ac-86f7-2fa592aaf2ad,
abstract = {{Suitable conditions for separating cells using a chromatographic procedure were evaluated in parallel chromatography on minicolumns. A 96-hole minicolumn plate filled with cryogel monoliths (18.8 mm x 7.1 mm O) with immobilized concanavalin A was used. Chromatographic columns (113 mm x 7.1 mm O) were used for chromatographic resolution of a mixture of Saccharomyces cerevisiae and Escherichia coli cells. Separation of a cell mixture containing equal amounts of cells of both types performed in a column format under the determined optimal conditions, resulted in a quantitative capture of applied S. cerevisiae cells, while E. coli passed through the column. Bound S. cerevisiae cells were released by flow-induced detachment and by compression of the adsorbent in the presence of 0.3 M methyl U-D-manno-pyranoside. The flowthrough and the eluted fractions were analyzed by plate counting and by registering metabolic activity of S. cerevisiae cells in the eluted fractions after capturing on ConA-cryogel monoliths in a 96-minicolumn plate format. The flowthrough fraction contained E. coli cells with nearly 100% purity, whereas the fraction eluted by compression of the adsorbent contained viable S. cerevisiae cells with 95% purity. Thus, an efficient chromatographic separation of cells was achieved using affinity cryogel column.}},
author = {{Dainiak, Maria and Galaev, Igor and Mattiasson, Bo}},
issn = {{0021-9673}},
keywords = {{screening; column format; format; 96-minicolumn plate; elastic; ConA-cryogel monoliths; macroporous; cell chromatography; cell detachment; compression of the adsorbent}},
language = {{eng}},
number = {{2}},
pages = {{145--150}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells}},
url = {{http://dx.doi.org/10.1016/j.chroma.2006.05.089}},
doi = {{10.1016/j.chroma.2006.05.089}},
volume = {{1123}},
year = {{2006}},
}