Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus Trametes versicolor PRL 572
(2002) In Applied and Environmental Microbiology 68(4). p.2077-2080- Abstract
- A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through... (More)
- A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1022809
- author
- Johansson, Tomas LU ; Nyman, Per-Olof LU and Cullen, Daniel
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 68
- issue
- 4
- pages
- 2077 - 2080
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0036211084
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.68.4.2077-2080.2002
- language
- English
- LU publication?
- yes
- id
- 3976b668-3848-428b-8356-d19de1c21c2e (old id 1022809)
- date added to LUP
- 2016-04-01 12:05:40
- date last changed
- 2024-01-08 08:10:55
@article{3976b668-3848-428b-8356-d19de1c21c2e,
abstract = {{A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology.}},
author = {{Johansson, Tomas and Nyman, Per-Olof and Cullen, Daniel}},
issn = {{0099-2240}},
language = {{eng}},
number = {{4}},
pages = {{2077--2080}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Differential regulation of mnp2, a new manganese peroxidase-encoding gene from the ligninolytic fungus Trametes versicolor PRL 572}},
url = {{http://dx.doi.org/10.1128/AEM.68.4.2077-2080.2002}},
doi = {{10.1128/AEM.68.4.2077-2080.2002}},
volume = {{68}},
year = {{2002}},
}