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Rapana thomasiana hemocyanin (RtH): Comparison of the two isoforms, RtH1 and RtH2, at 19 angstrom 16 angstrom resolution

Cheng, Kimberley ; Koeck, Philip J. B. ; Elmlund, Hans LU ; Idakieva, Krassimira ; Parvanova, Katja ; Schwarz, Heinz ; Ternström, Tomas LU and Hebert, Hans (2006) In Micron 37(6). p.566-576
Abstract
Three-dimensional (3D) reconstructions of the two 8.4 MDa Rapana thomasiana hemocyanin isoforms, RtH1 and RtH2, have been obtained by cryoelectron microscopy of molecules embedded in vitreous ice and single particle image processing. The final 3D structures of the RtH1 and RtH2 didecamers at 19 angstrom and 16 angstrom resolution, respectively, are very similar to earlier reconstructions of gastropodan hemocyanins, revealing structural features such as the obliquely oriented subunits, the five- and two-fold symmetrical axes. Three new interactions are defined; two of them connecting the arch and the wall while the third is formed between the collar and the wall. The collar-wall connection and one of the arch-wall connections are positioned... (More)
Three-dimensional (3D) reconstructions of the two 8.4 MDa Rapana thomasiana hemocyanin isoforms, RtH1 and RtH2, have been obtained by cryoelectron microscopy of molecules embedded in vitreous ice and single particle image processing. The final 3D structures of the RtH1 and RtH2 didecamers at 19 angstrom and 16 angstrom resolution, respectively, are very similar to earlier reconstructions of gastropodan hemocyanins, revealing structural features such as the obliquely oriented subunits, the five- and two-fold symmetrical axes. Three new interactions are defined; two of them connecting the arch and the wall while the third is formed between the collar and the wall. The collar-wall connection and one of the arch-wall connections are positioned between two individual subunit dimers, while the second arch-wall connection is located between two subunits within the subunit dimer. All three interactions establish connections to the first tier of the wall. Furthermore, for each interaction we have allocated two first tier functional units most likely involved in forming the connections. ( (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cryoelectron microscopy, Rapana thomasiana, hemocyanin, mollusc
in
Micron
volume
37
issue
6
pages
566 - 576
publisher
Elsevier
external identifiers
  • pmid:16466927
  • wos:000239696200009
  • pmid:16466927
  • scopus:33745958772
ISSN
0968-4328
DOI
10.1016/j.micron.2005.11.014
language
English
LU publication?
yes
id
5eff1cec-6658-4627-8053-4534a47099d9 (old id 398335)
date added to LUP
2016-04-01 11:52:34
date last changed
2021-02-17 02:49:32
@article{5eff1cec-6658-4627-8053-4534a47099d9,
  abstract     = {Three-dimensional (3D) reconstructions of the two 8.4 MDa Rapana thomasiana hemocyanin isoforms, RtH1 and RtH2, have been obtained by cryoelectron microscopy of molecules embedded in vitreous ice and single particle image processing. The final 3D structures of the RtH1 and RtH2 didecamers at 19 angstrom and 16 angstrom resolution, respectively, are very similar to earlier reconstructions of gastropodan hemocyanins, revealing structural features such as the obliquely oriented subunits, the five- and two-fold symmetrical axes. Three new interactions are defined; two of them connecting the arch and the wall while the third is formed between the collar and the wall. The collar-wall connection and one of the arch-wall connections are positioned between two individual subunit dimers, while the second arch-wall connection is located between two subunits within the subunit dimer. All three interactions establish connections to the first tier of the wall. Furthermore, for each interaction we have allocated two first tier functional units most likely involved in forming the connections. (},
  author       = {Cheng, Kimberley and Koeck, Philip J. B. and Elmlund, Hans and Idakieva, Krassimira and Parvanova, Katja and Schwarz, Heinz and Ternström, Tomas and Hebert, Hans},
  issn         = {0968-4328},
  language     = {eng},
  number       = {6},
  pages        = {566--576},
  publisher    = {Elsevier},
  series       = {Micron},
  title        = {Rapana thomasiana hemocyanin (RtH): Comparison of the two isoforms, RtH1 and RtH2, at 19 angstrom 16 angstrom resolution},
  url          = {http://dx.doi.org/10.1016/j.micron.2005.11.014},
  doi          = {10.1016/j.micron.2005.11.014},
  volume       = {37},
  year         = {2006},
}