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Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Nitsawang, Sarote ; Hatti-Kaul, Rajni LU and Kanasawuda, Pawinee (2006) In Enzyme and Microbial Technology 39(5). p.1103-1107
Abstract
Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. (
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
salt precipitation, aqueous two-phase extraction, Carica papaya latex, papain, two-step
in
Enzyme and Microbial Technology
volume
39
issue
5
pages
1103 - 1107
publisher
Elsevier
external identifiers
  • wos:000239694500020
  • scopus:33745959045
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.02.013
language
English
LU publication?
yes
id
379ee416-b2f6-4a43-826d-cdf5bb6174a1 (old id 398446)
date added to LUP
2016-04-01 12:02:27
date last changed
2020-08-05 01:54:09
@article{379ee416-b2f6-4a43-826d-cdf5bb6174a1,
  abstract     = {Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. (},
  author       = {Nitsawang, Sarote and Hatti-Kaul, Rajni and Kanasawuda, Pawinee},
  issn         = {0141-0229},
  language     = {eng},
  number       = {5},
  pages        = {1103--1107},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.02.013},
  doi          = {10.1016/j.enzmictec.2006.02.013},
  volume       = {39},
  year         = {2006},
}