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Synthesis, regulation and production of urokinase using mammalian cell culture: A comprehensive review

Roychoudhury, Pradip ; Khaparde, Shilpa ; Mattiasson, Bo LU and Kumar, Ashok LU (2006) In Biotechnology Advances 24(5). p.514-528
Abstract
Urokinase, a serine protease, catalyzes the conversion of plasminogen to plasmin, which is responsible for dissolution of clots in blood vessels. It is an important drug for treatment of thromboembolic disease. Production of urokinase by mammalian cell culture has the following important steps: synthesis, regulation and secretion. Production and accumulation of this product in a bioreactor is a real challenge for biochemical engineers. Considerable information at molecular level needs to be understood for production of urokinase in order to correlate different parameters, which in turn can maximize the productivity. This information will be highlighted in this review. Moreover, urokinase production is a product-inhibited process.... (More)
Urokinase, a serine protease, catalyzes the conversion of plasminogen to plasmin, which is responsible for dissolution of clots in blood vessels. It is an important drug for treatment of thromboembolic disease. Production of urokinase by mammalian cell culture has the following important steps: synthesis, regulation and secretion. Production and accumulation of this product in a bioreactor is a real challenge for biochemical engineers. Considerable information at molecular level needs to be understood for production of urokinase in order to correlate different parameters, which in turn can maximize the productivity. This information will be highlighted in this review. Moreover, urokinase production is a product-inhibited process. Therefore, in situ urokinase separation strategy is required to operate a bioreactor at its maximum urokinase formation rate. Integrated urokinase production and isolation processes developed recently will also be discussed briefly in this review. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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Contribution to journal
publication status
published
subject
keywords
plasminogen activator, tissue, urokinase synthesis and regulation, urokinase production, process integration
in
Biotechnology Advances
volume
24
issue
5
pages
514 - 528
publisher
Elsevier
external identifiers
  • wos:000239705600006
  • scopus:33746267955
ISSN
0734-9750
DOI
10.1016/j.biotechadv.2006.05.002
language
English
LU publication?
yes
id
9e0f3c05-4c1f-4b0c-80f2-fa595d7bae3f (old id 398511)
date added to LUP
2016-04-01 15:32:26
date last changed
2021-10-06 05:17:48
@article{9e0f3c05-4c1f-4b0c-80f2-fa595d7bae3f,
  abstract     = {Urokinase, a serine protease, catalyzes the conversion of plasminogen to plasmin, which is responsible for dissolution of clots in blood vessels. It is an important drug for treatment of thromboembolic disease. Production of urokinase by mammalian cell culture has the following important steps: synthesis, regulation and secretion. Production and accumulation of this product in a bioreactor is a real challenge for biochemical engineers. Considerable information at molecular level needs to be understood for production of urokinase in order to correlate different parameters, which in turn can maximize the productivity. This information will be highlighted in this review. Moreover, urokinase production is a product-inhibited process. Therefore, in situ urokinase separation strategy is required to operate a bioreactor at its maximum urokinase formation rate. Integrated urokinase production and isolation processes developed recently will also be discussed briefly in this review. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Roychoudhury, Pradip and Khaparde, Shilpa and Mattiasson, Bo and Kumar, Ashok},
  issn         = {0734-9750},
  language     = {eng},
  number       = {5},
  pages        = {514--528},
  publisher    = {Elsevier},
  series       = {Biotechnology Advances},
  title        = {Synthesis, regulation and production of urokinase using mammalian cell culture: A comprehensive review},
  url          = {http://dx.doi.org/10.1016/j.biotechadv.2006.05.002},
  doi          = {10.1016/j.biotechadv.2006.05.002},
  volume       = {24},
  year         = {2006},
}