Fractionation of wheat proteins by counter-current distribution using aqueous two-phase systems containing propionic acid
(1996) In Journal of Chromatography. B 680(1-2). p.71-80- Abstract
- Wheat proteins, soluble in diluted acid (glutenins), have been fractionated by counter-current distribution (CCD) using an aqueous two-phase system. The phase system is based on poly(ethylene glycol) and dextran but contains also 1% propionic acid and 6 mM magnesium sulfate. Approximately half of the bulk proteins partitioned to the upper phase while starch and other particles were recovered only into the lower phase. Whole wheat flour could be applied as sample for the CCD and 57 transfers were carried out. Starch and insoluble proteins remained stationary, while proteins followed the mobile phase to various degrees giving rise to a distribution pattern. The CCD pattern of the proteins showed distinct differences when various kinds of... (More)
- Wheat proteins, soluble in diluted acid (glutenins), have been fractionated by counter-current distribution (CCD) using an aqueous two-phase system. The phase system is based on poly(ethylene glycol) and dextran but contains also 1% propionic acid and 6 mM magnesium sulfate. Approximately half of the bulk proteins partitioned to the upper phase while starch and other particles were recovered only into the lower phase. Whole wheat flour could be applied as sample for the CCD and 57 transfers were carried out. Starch and insoluble proteins remained stationary, while proteins followed the mobile phase to various degrees giving rise to a distribution pattern. The CCD pattern of the proteins showed distinct differences when various kinds of wheat flour were analysed. The patterns indicate that at least six subpopulations of proteins can be obtained by using two-phase extraction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126365
- author
- Truust, Helle and Johansson, Göte
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Propionic acid, Proteins, Aqueous two-phase systems
- in
- Journal of Chromatography. B
- volume
- 680
- issue
- 1-2
- pages
- 71 - 80
- publisher
- Elsevier
- external identifiers
-
- scopus:0030014994
- ISSN
- 1572-6495
- DOI
- 10.1016/0378-4347(95)00539-0
- language
- English
- LU publication?
- no
- id
- 3990172e-612e-4a0c-8e73-602c6d198e53 (old id 126365)
- date added to LUP
- 2016-04-01 11:59:55
- date last changed
- 2022-01-26 21:22:46
@article{3990172e-612e-4a0c-8e73-602c6d198e53,
abstract = {{Wheat proteins, soluble in diluted acid (glutenins), have been fractionated by counter-current distribution (CCD) using an aqueous two-phase system. The phase system is based on poly(ethylene glycol) and dextran but contains also 1% propionic acid and 6 mM magnesium sulfate. Approximately half of the bulk proteins partitioned to the upper phase while starch and other particles were recovered only into the lower phase. Whole wheat flour could be applied as sample for the CCD and 57 transfers were carried out. Starch and insoluble proteins remained stationary, while proteins followed the mobile phase to various degrees giving rise to a distribution pattern. The CCD pattern of the proteins showed distinct differences when various kinds of wheat flour were analysed. The patterns indicate that at least six subpopulations of proteins can be obtained by using two-phase extraction.}},
author = {{Truust, Helle and Johansson, Göte}},
issn = {{1572-6495}},
keywords = {{Propionic acid; Proteins; Aqueous two-phase systems}},
language = {{eng}},
number = {{1-2}},
pages = {{71--80}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography. B}},
title = {{Fractionation of wheat proteins by counter-current distribution using aqueous two-phase systems containing propionic acid}},
url = {{http://dx.doi.org/10.1016/0378-4347(95)00539-0}},
doi = {{10.1016/0378-4347(95)00539-0}},
volume = {{680}},
year = {{1996}},
}