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Novel method to prepare morphologically rich polymeric surfaces for biomedical applications via phase separation and arrest of microgel particles

Lynch, Iseult LU ; Miller, Ian ; Gallagher, William M. and Dawson, Kenneth A. (2006) In The Journal of Physical Chemistry Part B 110(30). p.14581-14589
Abstract
We outline here a simple method to prepare polymeric surfaces of controlled surface topography on the micrometer scale, via assembly and arrest of microgel particles, for use in a range of biological applications to modify cell adhesion and spreading. In previous work by other groups, it has transpired that topography on the nanoscale is unlikely to be useful for this purpose, as roughness on this scale is often covered or coated by serum derived proteins during the early stages of cell adhesion and cells can easily bridge nanoscale roughness. Therefore, in our work, we have focused on roughness or topographic variations on the micrometer length scale. The basic idea is to modify the interactions between particles, thereby causing the... (More)
We outline here a simple method to prepare polymeric surfaces of controlled surface topography on the micrometer scale, via assembly and arrest of microgel particles, for use in a range of biological applications to modify cell adhesion and spreading. In previous work by other groups, it has transpired that topography on the nanoscale is unlikely to be useful for this purpose, as roughness on this scale is often covered or coated by serum derived proteins during the early stages of cell adhesion and cells can easily bridge nanoscale roughness. Therefore, in our work, we have focused on roughness or topographic variations on the micrometer length scale. The basic idea is to modify the interactions between particles, thereby causing the microgel particles to phase separate into particle-dense and particle-dilute domains and to arrest these domains on the surface. The result is the creation of surfaces with controlled topography. By changing the particle size, it is possible to alter the size of the pores formed and their distribution in the film. Preliminary results show that the system can readily be arrested into a homologous series of such structures (formed from microgel particles of the same size and same chemical structure) with biological implications. At the extremes of this series, large phenotypic differences are observed between cells, ranging (at one end) from localization of the cells in the pores to (at the other end) cells that avoid such localization, and remain extended, growing along the ridges between the pores. This constitutes a sort of cell localization transition on a surface with identical chemical components, where only the morphology has been adjusted. (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
110
issue
30
pages
14581 - 14589
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000239309500009
  • scopus:33748290078
  • pmid:16869558
ISSN
1520-5207
DOI
10.1021/jp061166a
language
English
LU publication?
yes
id
0deacccb-896f-4240-98c9-27f9c9603264 (old id 399465)
date added to LUP
2016-04-01 16:09:48
date last changed
2021-05-19 03:42:22
@article{0deacccb-896f-4240-98c9-27f9c9603264,
  abstract     = {We outline here a simple method to prepare polymeric surfaces of controlled surface topography on the micrometer scale, via assembly and arrest of microgel particles, for use in a range of biological applications to modify cell adhesion and spreading. In previous work by other groups, it has transpired that topography on the nanoscale is unlikely to be useful for this purpose, as roughness on this scale is often covered or coated by serum derived proteins during the early stages of cell adhesion and cells can easily bridge nanoscale roughness. Therefore, in our work, we have focused on roughness or topographic variations on the micrometer length scale. The basic idea is to modify the interactions between particles, thereby causing the microgel particles to phase separate into particle-dense and particle-dilute domains and to arrest these domains on the surface. The result is the creation of surfaces with controlled topography. By changing the particle size, it is possible to alter the size of the pores formed and their distribution in the film. Preliminary results show that the system can readily be arrested into a homologous series of such structures (formed from microgel particles of the same size and same chemical structure) with biological implications. At the extremes of this series, large phenotypic differences are observed between cells, ranging (at one end) from localization of the cells in the pores to (at the other end) cells that avoid such localization, and remain extended, growing along the ridges between the pores. This constitutes a sort of cell localization transition on a surface with identical chemical components, where only the morphology has been adjusted.},
  author       = {Lynch, Iseult and Miller, Ian and Gallagher, William M. and Dawson, Kenneth A.},
  issn         = {1520-5207},
  language     = {eng},
  number       = {30},
  pages        = {14581--14589},
  publisher    = {The American Chemical Society (ACS)},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Novel method to prepare morphologically rich polymeric surfaces for biomedical applications via phase separation and arrest of microgel particles},
  url          = {http://dx.doi.org/10.1021/jp061166a},
  doi          = {10.1021/jp061166a},
  volume       = {110},
  year         = {2006},
}