Macro/Nano-Structured Silicon as Solid Support for Antibody Arrays: Surface Design, Reproducibility, and Assay Characteristics Enabling Discovery of Kallikrein Gene Products for Prostate Disease Diagnostics
Ressine, Anton LU ; Finnskog, David LU ; Malm, Johan LU ; Becker, Charlotte LU ; Lilja, Hans LU
; Marko Varga, G
and Laurell, T
(2005)
In Nanobiotechnology
1(1).
p.93-104
- Abstract
- To facilitate high-throughput biomarker discovery and high-density protein-chip array analyses of complex biological samples, a novel macro- and nanoporous silicon surface for protein microarrays was developed. The surface offers three-dimensional surface enlarging properties and spot confinement, enabling both high sensitivity bioassays and design of high density arrays. Reproducible manufacturing of the protein chip surface was accomplished as demonstrated by the low imprecision when standard IgG bioassays were performed at 100 pM antigen level on a series of protein chips scanned at widely different locations within a silicon wafer, as well as between different wafers from two different manufacturers. The relative standard deviation... (More)
- To facilitate high-throughput biomarker discovery and high-density protein-chip array analyses of complex biological samples, a novel macro- and nanoporous silicon surface for protein microarrays was developed. The surface offers three-dimensional surface enlarging properties and spot confinement, enabling both high sensitivity bioassays and design of high density arrays. Reproducible manufacturing of the protein chip surface was accomplished as demonstrated by the low imprecision when standard IgG bioassays were performed at 100 pM antigen level on a series of protein chips scanned at widely different locations within a silicon wafer, as well as between different wafers from two different manufacturers. The relative standard deviation (RSD) of fluorescence spot intensity within an array on a chip was less than 20%. Mean spot intensity RSD was 19% for all 25 microarray chips in the study. Within-manufacturer-lot RSDs in chips from either manufacturer were <15% of mean spot intensity. The detection limit and dynamic range of the novel protein chip surface were examined to evaluate whether they match criteria required in a search for novel biomarkers such as for prostate cancer. Monoclonal IgG against prostate-specific antigen (PSA) was arrayed on the porous silicon chips. These were subsequently incubated in serum samples containing widely different levels of fluorescence-labeled PSA. Detection of PSA in serum at concentrations from 0.7 ng/mL (26 pM) up to 104-fold higher levels verified assay characteristics required in the search for prostate biomarkers (e.g., kallikrein gene products) at clinically relevant levels. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/744651
- author
- Ressine, Anton
LU
; Finnskog, David
LU
; Malm, Johan
LU
; Becker, Charlotte
LU
; Lilja, Hans
LU
; Marko Varga, G
and Laurell, T
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- macro/nano-structured silicon, biomarker discovery, Protein microarray, antibody
- in
- Nanobiotechnology
- volume
- 1
- issue
- 1
- pages
- 93 - 104
- publisher
- Humana Press
- external identifiers
-
- scopus:24644485923
- ISSN
- 1551-1286
- DOI
- 10.1385/NBT:1:1:093
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Clinical Chemistry, Malmö (013016000), Analytical Chemistry (S/LTH) (011001004)
- id
- 39afa59e-27c9-4c44-aea1-7368afbe8978 (old id 744651)
- alternative location
- http://www.springerlink.com/content/t737157u4220w536/fulltext.pdf
- date added to LUP
- 2016-04-01 16:58:33
- date last changed
- 2022-02-05 19:52:40
@article{39afa59e-27c9-4c44-aea1-7368afbe8978,
abstract = {{To facilitate high-throughput biomarker discovery and high-density protein-chip array analyses of complex biological samples, a novel macro- and nanoporous silicon surface for protein microarrays was developed. The surface offers three-dimensional surface enlarging properties and spot confinement, enabling both high sensitivity bioassays and design of high density arrays. Reproducible manufacturing of the protein chip surface was accomplished as demonstrated by the low imprecision when standard IgG bioassays were performed at 100 pM antigen level on a series of protein chips scanned at widely different locations within a silicon wafer, as well as between different wafers from two different manufacturers. The relative standard deviation (RSD) of fluorescence spot intensity within an array on a chip was less than 20%. Mean spot intensity RSD was 19% for all 25 microarray chips in the study. Within-manufacturer-lot RSDs in chips from either manufacturer were <15% of mean spot intensity. The detection limit and dynamic range of the novel protein chip surface were examined to evaluate whether they match criteria required in a search for novel biomarkers such as for prostate cancer. Monoclonal IgG against prostate-specific antigen (PSA) was arrayed on the porous silicon chips. These were subsequently incubated in serum samples containing widely different levels of fluorescence-labeled PSA. Detection of PSA in serum at concentrations from 0.7 ng/mL (26 pM) up to 104-fold higher levels verified assay characteristics required in the search for prostate biomarkers (e.g., kallikrein gene products) at clinically relevant levels.}},
author = {{Ressine, Anton and Finnskog, David and Malm, Johan and Becker, Charlotte and Lilja, Hans and Marko Varga, G and Laurell, T}},
issn = {{1551-1286}},
keywords = {{macro/nano-structured silicon; biomarker discovery; Protein microarray; antibody}},
language = {{eng}},
number = {{1}},
pages = {{93--104}},
publisher = {{Humana Press}},
series = {{Nanobiotechnology}},
title = {{Macro/Nano-Structured Silicon as Solid Support for Antibody Arrays: Surface Design, Reproducibility, and Assay Characteristics Enabling Discovery of Kallikrein Gene Products for Prostate Disease Diagnostics}},
url = {{http://dx.doi.org/10.1385/NBT:1:1:093}},
doi = {{10.1385/NBT:1:1:093}},
volume = {{1}},
year = {{2005}},
}