ACouWash : A standalone instrument for the washing, separation and enrichment of cells.
Mallinson, Jay ; Linander, Oskar ; Magnusson, Cecilia LU ; Pircs, Karolina LU
and Augustsson, Per
LU
(2018)
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
1.
p.279-281
- Abstract
We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing, cell viability, and time savings compared to centrifugation. We repeatedly recovered 90-100% of target cells and particles, washed away 99.9% of a small fluorescent tracer molecule, viability of cells was unchanged, and reduced the time for standard cell labelling by 50%.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3a180be0-a9c6-45a4-ba62-89602d93b097
- author
- Mallinson, Jay
; Linander, Oskar
; Magnusson, Cecilia
LU
; Pircs, Karolina
LU
and Augustsson, Per
LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustofluidics, Acoustophoresis, Blood cells, Enrichment, Separation
- host publication
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- series title
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- volume
- 1
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- conference location
- Kaohsiung, Taiwan
- conference dates
- 2018-11-11 - 2018-11-15
- ISBN
- 9781510897571
- language
- English
- LU publication?
- yes
- id
- 3a180be0-a9c6-45a4-ba62-89602d93b097
- date added to LUP
- 2020-03-13 15:43:20
- date last changed
- 2022-02-01 05:04:45
@inproceedings{3a180be0-a9c6-45a4-ba62-89602d93b097,
abstract = {{<p>We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing, cell viability, and time savings compared to centrifugation. We repeatedly recovered 90-100% of target cells and particles, washed away 99.9% of a small fluorescent tracer molecule, viability of cells was unchanged, and reduced the time for standard cell labelling by 50%.</p>}},
author = {{Mallinson, Jay and Linander, Oskar and Magnusson, Cecilia and Pircs, Karolina and Augustsson, Per}},
booktitle = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}},
isbn = {{9781510897571}},
keywords = {{Acoustofluidics; Acoustophoresis; Blood cells; Enrichment; Separation}},
language = {{eng}},
pages = {{279--281}},
publisher = {{Chemical and Biological Microsystems Society}},
series = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}},
title = {{ACouWash : A standalone instrument for the washing, separation and enrichment of cells.}},
volume = {{1}},
year = {{2018}},
}