Gelatin nanoparticle fabrication and optimization of the particle size
Jahanshahi, M. ; Sanati, Mohammad Hossein ; Hajizadeh, S. LU
and Babaei, Z.
(2008)
In Physica Status Solidi (A) Applications and Materials Science
205(12).
p.2898-2902
- Abstract
The biotechnology industry has recently been demanding second generation of nanoparticle byproducts such as viruses, plasmids, virus-like particles and drug-delivery assemblies (20-300 nm). The possibility of preparing uniform nanoparticles consisting of proteins such as gelatin followed by covalent linkage of avidin was investigated. Gelatin nanoparticles were prepared by two-step desolvation. As a colloidal drug-delivery system, the essential parameters in fabrication were optimized by the Taguchi design method. However, for characterizing the nanoparticles AFM and SEM were employed. By introducing 4 factors (temperature, gelatin concentration, agitation speed and the amount of acetone) in 4 levels to the software 16 experiments were... (More)
The biotechnology industry has recently been demanding second generation of nanoparticle byproducts such as viruses, plasmids, virus-like particles and drug-delivery assemblies (20-300 nm). The possibility of preparing uniform nanoparticles consisting of proteins such as gelatin followed by covalent linkage of avidin was investigated. Gelatin nanoparticles were prepared by two-step desolvation. As a colloidal drug-delivery system, the essential parameters in fabrication were optimized by the Taguchi design method. However, for characterizing the nanoparticles AFM and SEM were employed. By introducing 4 factors (temperature, gelatin concentration, agitation speed and the amount of acetone) in 4 levels to the software 16 experiments were carried out and the optimum condition was gained in 50 °C, 45 mg/ml gelatin concentration, 80 ml of acetone based on reduction of the size. The produced nanoparticles size was under 174 nm. The mechanistic of the optimum conditions for preparing protein nanoparticles as well as their characterization are discussed in detail.
(Less)
- author
- Jahanshahi, M.
; Sanati, Mohammad Hossein
; Hajizadeh, S.
LU
and Babaei, Z.
- publishing date
- 2008-12
- type
- Contribution to journal
- publication status
- published
- in
- Physica Status Solidi (A) Applications and Materials Science
- volume
- 205
- issue
- 12
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:57349099421
- ISSN
- 1862-6300
- DOI
- 10.1002/pssa.200824329
- language
- English
- LU publication?
- no
- id
- 3a4a4fa0-8b34-4048-b496-b89e0811ce00
- date added to LUP
- 2023-08-24 08:32:11
- date last changed
- 2023-08-28 14:15:39
@article{3a4a4fa0-8b34-4048-b496-b89e0811ce00,
abstract = {{<p>The biotechnology industry has recently been demanding second generation of nanoparticle byproducts such as viruses, plasmids, virus-like particles and drug-delivery assemblies (20-300 nm). The possibility of preparing uniform nanoparticles consisting of proteins such as gelatin followed by covalent linkage of avidin was investigated. Gelatin nanoparticles were prepared by two-step desolvation. As a colloidal drug-delivery system, the essential parameters in fabrication were optimized by the Taguchi design method. However, for characterizing the nanoparticles AFM and SEM were employed. By introducing 4 factors (temperature, gelatin concentration, agitation speed and the amount of acetone) in 4 levels to the software 16 experiments were carried out and the optimum condition was gained in 50 °C, 45 mg/ml gelatin concentration, 80 ml of acetone based on reduction of the size. The produced nanoparticles size was under 174 nm. The mechanistic of the optimum conditions for preparing protein nanoparticles as well as their characterization are discussed in detail.</p>}},
author = {{Jahanshahi, M. and Sanati, Mohammad Hossein and Hajizadeh, S. and Babaei, Z.}},
issn = {{1862-6300}},
language = {{eng}},
number = {{12}},
pages = {{2898--2902}},
publisher = {{Wiley-Blackwell}},
series = {{Physica Status Solidi (A) Applications and Materials Science}},
title = {{Gelatin nanoparticle fabrication and optimization of the particle size}},
url = {{http://dx.doi.org/10.1002/pssa.200824329}},
doi = {{10.1002/pssa.200824329}},
volume = {{205}},
year = {{2008}},
}