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Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.

Doi, Daisuke ; Samata, Bumpei ; Katsukawa, Mitsuko ; Kikuchi, Tetsuhiro ; Morizane, Asuka LU ; Ono, Yuichi ; Sekiguchi, Kiyotoshi ; Nakagawa, Masato ; Parmar, Malin LU orcid and Takahashi, Jun (2014) In Stem Cell Reports 2(3). p.337-350
Abstract
Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor... (More)
Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application. (Less)
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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Reports
volume
2
issue
3
pages
337 - 350
publisher
Cell Press
external identifiers
  • pmid:24672756
  • wos:000336647700009
  • scopus:84896084435
  • pmid:24672756
ISSN
2213-6711
DOI
10.1016/j.stemcr.2014.01.013
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Developmental Neurobiology (013210001), Neuronal Survival (013212041)
id
3a792abe-e9d7-485f-840f-be252c9d52d1 (old id 4379606)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24672756?dopt=Abstract
date added to LUP
2016-04-01 14:43:50
date last changed
2022-05-20 02:01:44
@article{3a792abe-e9d7-485f-840f-be252c9d52d1,
  abstract     = {{Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.}},
  author       = {{Doi, Daisuke and Samata, Bumpei and Katsukawa, Mitsuko and Kikuchi, Tetsuhiro and Morizane, Asuka and Ono, Yuichi and Sekiguchi, Kiyotoshi and Nakagawa, Masato and Parmar, Malin and Takahashi, Jun}},
  issn         = {{2213-6711}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{337--350}},
  publisher    = {{Cell Press}},
  series       = {{Stem Cell Reports}},
  title        = {{Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.}},
  url          = {{http://dx.doi.org/10.1016/j.stemcr.2014.01.013}},
  doi          = {{10.1016/j.stemcr.2014.01.013}},
  volume       = {{2}},
  year         = {{2014}},
}