Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.
(2014) In Stem Cell Reports 2(3). p.337-350- Abstract
- Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor... (More)
- Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4379606
- author
- Doi, Daisuke ; Samata, Bumpei ; Katsukawa, Mitsuko ; Kikuchi, Tetsuhiro ; Morizane, Asuka LU ; Ono, Yuichi ; Sekiguchi, Kiyotoshi ; Nakagawa, Masato ; Parmar, Malin LU and Takahashi, Jun
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Reports
- volume
- 2
- issue
- 3
- pages
- 337 - 350
- publisher
- Cell Press
- external identifiers
-
- pmid:24672756
- wos:000336647700009
- scopus:84896084435
- pmid:24672756
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2014.01.013
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Developmental Neurobiology (013210001), Neuronal Survival (013212041)
- id
- 3a792abe-e9d7-485f-840f-be252c9d52d1 (old id 4379606)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24672756?dopt=Abstract
- date added to LUP
- 2016-04-01 14:43:50
- date last changed
- 2022-05-20 02:01:44
@article{3a792abe-e9d7-485f-840f-be252c9d52d1, abstract = {{Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson's disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN(+) cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN(+) cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN(+) cells in a NURR1(+) cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.}}, author = {{Doi, Daisuke and Samata, Bumpei and Katsukawa, Mitsuko and Kikuchi, Tetsuhiro and Morizane, Asuka and Ono, Yuichi and Sekiguchi, Kiyotoshi and Nakagawa, Masato and Parmar, Malin and Takahashi, Jun}}, issn = {{2213-6711}}, language = {{eng}}, number = {{3}}, pages = {{337--350}}, publisher = {{Cell Press}}, series = {{Stem Cell Reports}}, title = {{Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.}}, url = {{http://dx.doi.org/10.1016/j.stemcr.2014.01.013}}, doi = {{10.1016/j.stemcr.2014.01.013}}, volume = {{2}}, year = {{2014}}, }