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Metabolic potential of the moderate halophile Yangia sp. ND199 for co-production of polyhydroxyalkanoates and exopolysaccharides

Romero Soto, Luis LU ; Thabet, Habib ; Maghembe, Reuben ; Gameiro, Denise ; Van-Thuoc, Doan ; Dishisha, Tarek LU and Hatti-Kaul, Rajni LU (2021) In MicrobiologyOpen 10(1).
Abstract

Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co-production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB-M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with... (More)

Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co-production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB-M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
co-production, exopolysaccharide, moderate halophile, poly(3-hydroxybutyrate), Yangia sp. ND199
in
MicrobiologyOpen
volume
10
issue
1
article number
e1160
publisher
Wiley-Blackwell
external identifiers
  • pmid:33650793
  • scopus:85102224368
ISSN
2045-8827
DOI
10.1002/mbo3.1160
language
English
LU publication?
yes
id
3a853c09-040a-4cfa-84d2-dba2a4220989
date added to LUP
2021-03-29 14:14:58
date last changed
2025-02-09 09:01:56
@article{3a853c09-040a-4cfa-84d2-dba2a4220989,
  abstract     = {{<p>Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co-production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB-M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.</p>}},
  author       = {{Romero Soto, Luis and Thabet, Habib and Maghembe, Reuben and Gameiro, Denise and Van-Thuoc, Doan and Dishisha, Tarek and Hatti-Kaul, Rajni}},
  issn         = {{2045-8827}},
  keywords     = {{co-production; exopolysaccharide; moderate halophile; poly(3-hydroxybutyrate); Yangia sp. ND199}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{MicrobiologyOpen}},
  title        = {{Metabolic potential of the moderate halophile Yangia sp. ND199 for co-production of polyhydroxyalkanoates and exopolysaccharides}},
  url          = {{http://dx.doi.org/10.1002/mbo3.1160}},
  doi          = {{10.1002/mbo3.1160}},
  volume       = {{10}},
  year         = {{2021}},
}