A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory
(2022) In Journal of Synchrotron Radiation 29. p.555-562- Abstract
The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that... (More)
The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.
(Less)
- author
- Berntsson, Oskar LU ; Terry, Ann E. LU and Plivelic, Tomás S. LU
- organization
- publishing date
- 2022-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- beamline, time-resolved SAXS, time-resolved X-ray solution scattering
- in
- Journal of Synchrotron Radiation
- volume
- 29
- pages
- 8 pages
- publisher
- International Union of Crystallography
- external identifiers
-
- pmid:35254321
- scopus:85125964612
- ISSN
- 0909-0495
- DOI
- 10.1107/S1600577522000996
- language
- English
- LU publication?
- yes
- id
- 3aa84791-6c7a-4bdc-9536-438f805e0d4a
- date added to LUP
- 2022-06-14 11:33:26
- date last changed
- 2024-09-19 16:34:29
@article{3aa84791-6c7a-4bdc-9536-438f805e0d4a, abstract = {{<p>The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.</p>}}, author = {{Berntsson, Oskar and Terry, Ann E. and Plivelic, Tomás S.}}, issn = {{0909-0495}}, keywords = {{beamline; time-resolved SAXS; time-resolved X-ray solution scattering}}, language = {{eng}}, month = {{03}}, pages = {{555--562}}, publisher = {{International Union of Crystallography}}, series = {{Journal of Synchrotron Radiation}}, title = {{A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory}}, url = {{http://dx.doi.org/10.1107/S1600577522000996}}, doi = {{10.1107/S1600577522000996}}, volume = {{29}}, year = {{2022}}, }