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A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory

Berntsson, Oskar LU ; Terry, Ann E. LU and Plivelic, Tomás S. LU (2022) In Journal of Synchrotron Radiation 29. p.555-562
Abstract

The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that... (More)

The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.

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Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beamline, time-resolved SAXS, time-resolved X-ray solution scattering
in
Journal of Synchrotron Radiation
volume
29
pages
8 pages
publisher
International Union of Crystallography
external identifiers
  • pmid:35254321
  • scopus:85125964612
ISSN
0909-0495
DOI
10.1107/S1600577522000996
language
English
LU publication?
yes
id
3aa84791-6c7a-4bdc-9536-438f805e0d4a
date added to LUP
2022-06-14 11:33:26
date last changed
2024-06-13 07:19:16
@article{3aa84791-6c7a-4bdc-9536-438f805e0d4a,
  abstract     = {{<p>The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.</p>}},
  author       = {{Berntsson, Oskar and Terry, Ann E. and Plivelic, Tomás S.}},
  issn         = {{0909-0495}},
  keywords     = {{beamline; time-resolved SAXS; time-resolved X-ray solution scattering}},
  language     = {{eng}},
  month        = {{03}},
  pages        = {{555--562}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory}},
  url          = {{http://dx.doi.org/10.1107/S1600577522000996}},
  doi          = {{10.1107/S1600577522000996}},
  volume       = {{29}},
  year         = {{2022}},
}