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HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals

Owerbach, David ; Lernmark, Åke LU ; Platz, Per ; Ryder, Lars P. ; Rask, Lars ; Peterson, Per A. and Ludvigsson, Johnny (1983) In Nature 303(5920). p.815-817
Abstract

The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response1-3 but several human diseases are also found to be associated with certain HLA-DR antigens4. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism1 associated with the β-chains6,7 which seem, from hybridization studies, to be encoded by several genes8,9. We have therefore used the β-chain cDNA probe, pDR-β-1 (refs 8, 10) to test... (More)

The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response1-3 but several human diseases are also found to be associated with certain HLA-DR antigens4. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism1 associated with the β-chains6,7 which seem, from hybridization studies, to be encoded by several genes8,9. We have therefore used the β-chain cDNA probe, pDR-β-1 (refs 8, 10) to test whether there are differences in hybridization pattern between DNA from healthy individuals and diabetic patients, after digestion with restriction endonucleases. Among the HLA-DR 4 and 3/4 individuals, the IDDM patients showed an increased frequency of a PstI 18 kilobase (kb) fragment. A BamHI 3.7 kb fragment, frequent among controls (30-40%), was rarely detected in the IDDM patients (0-2%). These differences may be related to susceptibility to develop the disease.

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published
in
Nature
volume
303
issue
5920
pages
3 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:6306468
  • scopus:0020593780
ISSN
0028-0836
DOI
10.1038/303815a0
language
English
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no
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3ae390bc-f3db-4d46-9136-3f89155d8ea4
date added to LUP
2019-09-16 12:50:18
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2020-01-13 02:22:17
@article{3ae390bc-f3db-4d46-9136-3f89155d8ea4,
  abstract     = {<p>The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response<sup>1-3</sup> but several human diseases are also found to be associated with certain HLA-DR antigens<sup>4</sup>. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism<sup>1</sup> associated with the β-chains<sup>6,7</sup> which seem, from hybridization studies, to be encoded by several genes<sup>8,9</sup>. We have therefore used the β-chain cDNA probe, pDR-β-1 (refs 8, 10) to test whether there are differences in hybridization pattern between DNA from healthy individuals and diabetic patients, after digestion with restriction endonucleases. Among the HLA-DR 4 and 3/4 individuals, the IDDM patients showed an increased frequency of a PstI 18 kilobase (kb) fragment. A BamHI 3.7 kb fragment, frequent among controls (30-40%), was rarely detected in the IDDM patients (0-2%). These differences may be related to susceptibility to develop the disease.</p>},
  author       = {Owerbach, David and Lernmark, Åke and Platz, Per and Ryder, Lars P. and Rask, Lars and Peterson, Per A. and Ludvigsson, Johnny},
  issn         = {0028-0836},
  language     = {eng},
  month        = {12},
  number       = {5920},
  pages        = {815--817},
  publisher    = {Nature Publishing Group},
  series       = {Nature},
  title        = {HLA-D region β-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals},
  url          = {http://dx.doi.org/10.1038/303815a0},
  doi          = {10.1038/303815a0},
  volume       = {303},
  year         = {1983},
}