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Tumor necrosis factor-α-mediated downregulation of the cystic fibrosis transmembrane conductance regulator drives pathological sphingosine-1-phosphate signaling in a mouse model of heart failure

Meissner, Anja LU ; Yang, Jingli; Kroetsch, Jeffrey T.; Sauvé, Meghan; Dax, Hendrik; Momen, M. Abdul; Noyan-Ashraf, M. Hossein; Heximer, Scott; Husain, Mansoor and Lidington, Darcy, et al. (2012) In Circulation 125(22). p.2739-2750
Abstract

Background-Sphingosine-1-phosphate (S1P) signaling is a central regulator of resistance artery tone. Therefore, S1P levels need to be tightly controlled through the delicate interplay of its generating enzyme sphingosine kinase 1 and its functional antagonist S1P phosphohydrolase-1. The intracellular localization of S1P phosphohydrolase-1 necessitates the import of extracellular S1P into the intracellular compartment before its degradation. The present investigation proposes that the cystic fibrosis transmembrane conductance regulator transports extracellular S1P and hence modulates microvascular S1P signaling in health and disease. Methods and Results-In cultured murine vascular smooth muscle cells in vitro and isolated murine... (More)

Background-Sphingosine-1-phosphate (S1P) signaling is a central regulator of resistance artery tone. Therefore, S1P levels need to be tightly controlled through the delicate interplay of its generating enzyme sphingosine kinase 1 and its functional antagonist S1P phosphohydrolase-1. The intracellular localization of S1P phosphohydrolase-1 necessitates the import of extracellular S1P into the intracellular compartment before its degradation. The present investigation proposes that the cystic fibrosis transmembrane conductance regulator transports extracellular S1P and hence modulates microvascular S1P signaling in health and disease. Methods and Results-In cultured murine vascular smooth muscle cells in vitro and isolated murine mesenteric and posterior cerebral resistance arteries ex vivo, the cystic fibrosis transmembrane conductance regulator (1) is critical for S1P uptake; (2) modulates S1P-dependent responses; and (3) is downregulated in vitro and in vivo by tumor necrosis factor-α, with significant functional consequences for S1P signaling and vascular tone. In heart failure, tumor necrosis factor-α downregulates the cystic fibrosis transmembrane conductance regulator across several organs, including the heart, lung, and brain, suggesting that it is a fundamental mechanism with implications for systemic S1P effects. Conclusions-We identify the cystic fibrosis transmembrane conductance regulator as a critical regulatory site for S1P signaling; its tumor necrosis factor-α-dependent downregulation in heart failure underlies an enhancement in microvascular tone. This molecular mechanism potentially represents a novel and highly strategic therapeutic target for cardiovascular conditions involving inflammation.

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publishing date
type
Contribution to journal
publication status
published
keywords
Acute myocardial infarction, Hemodynamics, Myogenic vasoconstriction, Signal transduction, Vasomotor tone
in
Circulation
volume
125
issue
22
pages
12 pages
publisher
Lippincott Williams and Wilkins
external identifiers
  • scopus:84861854722
ISSN
0009-7322
DOI
10.1161/CIRCULATIONAHA.111.047316
language
English
LU publication?
no
id
3b1bc563-8739-4780-96b9-3ddebab02d7e
date added to LUP
2017-05-23 22:24:07
date last changed
2017-06-19 15:56:08
@article{3b1bc563-8739-4780-96b9-3ddebab02d7e,
  abstract     = {<p>Background-Sphingosine-1-phosphate (S1P) signaling is a central regulator of resistance artery tone. Therefore, S1P levels need to be tightly controlled through the delicate interplay of its generating enzyme sphingosine kinase 1 and its functional antagonist S1P phosphohydrolase-1. The intracellular localization of S1P phosphohydrolase-1 necessitates the import of extracellular S1P into the intracellular compartment before its degradation. The present investigation proposes that the cystic fibrosis transmembrane conductance regulator transports extracellular S1P and hence modulates microvascular S1P signaling in health and disease. Methods and Results-In cultured murine vascular smooth muscle cells in vitro and isolated murine mesenteric and posterior cerebral resistance arteries ex vivo, the cystic fibrosis transmembrane conductance regulator (1) is critical for S1P uptake; (2) modulates S1P-dependent responses; and (3) is downregulated in vitro and in vivo by tumor necrosis factor-α, with significant functional consequences for S1P signaling and vascular tone. In heart failure, tumor necrosis factor-α downregulates the cystic fibrosis transmembrane conductance regulator across several organs, including the heart, lung, and brain, suggesting that it is a fundamental mechanism with implications for systemic S1P effects. Conclusions-We identify the cystic fibrosis transmembrane conductance regulator as a critical regulatory site for S1P signaling; its tumor necrosis factor-α-dependent downregulation in heart failure underlies an enhancement in microvascular tone. This molecular mechanism potentially represents a novel and highly strategic therapeutic target for cardiovascular conditions involving inflammation.</p>},
  author       = {Meissner, Anja and Yang, Jingli and Kroetsch, Jeffrey T. and Sauvé, Meghan and Dax, Hendrik and Momen, M. Abdul and Noyan-Ashraf, M. Hossein and Heximer, Scott and Husain, Mansoor and Lidington, Darcy and Bolz, Steffen Sebastian},
  issn         = {0009-7322},
  keyword      = {Acute myocardial infarction,Hemodynamics,Myogenic vasoconstriction,Signal transduction,Vasomotor tone},
  language     = {eng},
  month        = {06},
  number       = {22},
  pages        = {2739--2750},
  publisher    = {Lippincott Williams and Wilkins},
  series       = {Circulation},
  title        = {Tumor necrosis factor-α-mediated downregulation of the cystic fibrosis transmembrane conductance regulator drives pathological sphingosine-1-phosphate signaling in a mouse model of heart failure},
  url          = {http://dx.doi.org/10.1161/CIRCULATIONAHA.111.047316},
  volume       = {125},
  year         = {2012},
}