Mast Cell Proteases Tryptase and Chymase Induce Migratory and Morphological Alterations in Bronchial Epithelial Cells
Berlin, Frida LU ; Mogren, Sofia LU ; Tutzauer, Julia LU
and Andersson, Cecilia
LU
(2021)
In International Journal of Molecular Sciences
22(10).
- Abstract
- Chronic respiratory diseases are often characterized by impaired epithelial function and remodeling. Mast cells (MCs) are known to home into the epithelium in respiratory diseases, but the MC-epithelial interactions remain less understood. Therefore, this study aimed to investigate the effect of MC proteases on bronchial epithelial morphology and function. Bronchial epithelial cells were stimulated with MC tryptase and/or chymase. Morphology and epithelial function were performed using cell tracking analysis and holographic live-cell imaging. Samples were also analyzed for motility-associated gene expression. Immunocytochemistry was performed to compare cytoskeletal arrangement. Stimulated cells showed strong alterations on gene, protein... (More)
- Chronic respiratory diseases are often characterized by impaired epithelial function and remodeling. Mast cells (MCs) are known to home into the epithelium in respiratory diseases, but the MC-epithelial interactions remain less understood. Therefore, this study aimed to investigate the effect of MC proteases on bronchial epithelial morphology and function. Bronchial epithelial cells were stimulated with MC tryptase and/or chymase. Morphology and epithelial function were performed using cell tracking analysis and holographic live-cell imaging. Samples were also analyzed for motility-associated gene expression. Immunocytochemistry was performed to compare cytoskeletal arrangement. Stimulated cells showed strong alterations on gene, protein and functional levels in several parameters important for maintaining epithelial function. The most significant increases were found in cell motility, cellular speed and cell elongation compared to non-stimulated cells. Also, cell morphology was significantly altered in chymase treated compared to non-stimulated cells. In the current study, we show that MC proteases can induce cell migration and morphological and proliferative alterations in epithelial cells. Thus, our data imply that MC release of proteases may play a critical role in airway epithelial remodeling and disruption of epithelial function. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3b50192f-948b-409e-974e-6a9287d72f96
- author
- Berlin, Frida
LU
; Mogren, Sofia
LU
; Tutzauer, Julia
LU
and Andersson, Cecilia
LU
- organization
- publishing date
- 2021-05-16
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- mast cell, tryptase, chymase, Bronchial epithelial cells, morphology, migration, proliferation, holomonitor
- in
- International Journal of Molecular Sciences
- volume
- 22
- issue
- 10
- article number
- 5250
- pages
- 20 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85105741517
- pmid:34065716
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms22105250
- language
- English
- LU publication?
- yes
- id
- 3b50192f-948b-409e-974e-6a9287d72f96
- date added to LUP
- 2021-05-31 19:21:39
- date last changed
- 2023-04-02 07:29:44
@article{3b50192f-948b-409e-974e-6a9287d72f96,
abstract = {{Chronic respiratory diseases are often characterized by impaired epithelial function and remodeling. Mast cells (MCs) are known to home into the epithelium in respiratory diseases, but the MC-epithelial interactions remain less understood. Therefore, this study aimed to investigate the effect of MC proteases on bronchial epithelial morphology and function. Bronchial epithelial cells were stimulated with MC tryptase and/or chymase. Morphology and epithelial function were performed using cell tracking analysis and holographic live-cell imaging. Samples were also analyzed for motility-associated gene expression. Immunocytochemistry was performed to compare cytoskeletal arrangement. Stimulated cells showed strong alterations on gene, protein and functional levels in several parameters important for maintaining epithelial function. The most significant increases were found in cell motility, cellular speed and cell elongation compared to non-stimulated cells. Also, cell morphology was significantly altered in chymase treated compared to non-stimulated cells. In the current study, we show that MC proteases can induce cell migration and morphological and proliferative alterations in epithelial cells. Thus, our data imply that MC release of proteases may play a critical role in airway epithelial remodeling and disruption of epithelial function.}},
author = {{Berlin, Frida and Mogren, Sofia and Tutzauer, Julia and Andersson, Cecilia}},
issn = {{1422-0067}},
keywords = {{mast cell; tryptase; chymase; Bronchial epithelial cells; morphology; migration; proliferation; holomonitor}},
language = {{eng}},
month = {{05}},
number = {{10}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Mast Cell Proteases Tryptase and Chymase Induce Migratory and Morphological Alterations in Bronchial Epithelial Cells}},
url = {{http://dx.doi.org/10.3390/ijms22105250}},
doi = {{10.3390/ijms22105250}},
volume = {{22}},
year = {{2021}},
}