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Simultaneous native UV fluorescence and absorbance detection for microchip electrophoresis using integrated waveguides

Ohlsson, P. D. LU orcid ; Ordeig, O. ; Mogensen, K. B. and Kutter, J. P. (2008) 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 p.1618-1620
Abstract

This paper reports simultaneous detection of ultra violet (UV) absorbance and native UV-excited fluorescence in an electrophoresis microchip using UV transparent integrated waveguides. Absorbance detection enables detection of a wide range of analytes. When exposed to UV light, some compounds also exhibit native fluorescence, which is here detected simultaneously. Calibration curves were measured for serotonin, D-tryptophan and propanolol and separation of the three compounds was performed. All compounds could be detected by both methods, but with a lower limit of detection for native fluorescence.

Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Absorbance, Electrophoresis, Fluorescence detection, Waveguides
host publication
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
conference location
San Diego, CA, United States
conference dates
2008-10-12 - 2008-10-16
external identifiers
  • scopus:84902438927
language
English
LU publication?
no
id
3b629621-4c1f-4f51-96a9-14eec3245116
date added to LUP
2017-12-06 15:14:13
date last changed
2022-01-31 00:22:48
@inproceedings{3b629621-4c1f-4f51-96a9-14eec3245116,
  abstract     = {{<p>This paper reports simultaneous detection of ultra violet (UV) absorbance and native UV-excited fluorescence in an electrophoresis microchip using UV transparent integrated waveguides. Absorbance detection enables detection of a wide range of analytes. When exposed to UV light, some compounds also exhibit native fluorescence, which is here detected simultaneously. Calibration curves were measured for serotonin, D-tryptophan and propanolol and separation of the three compounds was performed. All compounds could be detected by both methods, but with a lower limit of detection for native fluorescence.</p>}},
  author       = {{Ohlsson, P. D. and Ordeig, O. and Mogensen, K. B. and Kutter, J. P.}},
  booktitle    = {{12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference}},
  keywords     = {{Absorbance; Electrophoresis; Fluorescence detection; Waveguides}},
  language     = {{eng}},
  pages        = {{1618--1620}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{Simultaneous native UV fluorescence and absorbance detection for microchip electrophoresis using integrated waveguides}},
  year         = {{2008}},
}