Simultaneous native UV fluorescence and absorbance detection for microchip electrophoresis using integrated waveguides
(2008) 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 p.1618-1620- Abstract
This paper reports simultaneous detection of ultra violet (UV) absorbance and native UV-excited fluorescence in an electrophoresis microchip using UV transparent integrated waveguides. Absorbance detection enables detection of a wide range of analytes. When exposed to UV light, some compounds also exhibit native fluorescence, which is here detected simultaneously. Calibration curves were measured for serotonin, D-tryptophan and propanolol and separation of the three compounds was performed. All compounds could be detected by both methods, but with a lower limit of detection for native fluorescence.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3b629621-4c1f-4f51-96a9-14eec3245116
- author
- Ohlsson, P. D. LU ; Ordeig, O. ; Mogensen, K. B. and Kutter, J. P.
- publishing date
- 2008
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Absorbance, Electrophoresis, Fluorescence detection, Waveguides
- host publication
- 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
- conference location
- San Diego, CA, United States
- conference dates
- 2008-10-12 - 2008-10-16
- external identifiers
-
- scopus:84902438927
- language
- English
- LU publication?
- no
- id
- 3b629621-4c1f-4f51-96a9-14eec3245116
- date added to LUP
- 2017-12-06 15:14:13
- date last changed
- 2022-01-31 00:22:48
@inproceedings{3b629621-4c1f-4f51-96a9-14eec3245116, abstract = {{<p>This paper reports simultaneous detection of ultra violet (UV) absorbance and native UV-excited fluorescence in an electrophoresis microchip using UV transparent integrated waveguides. Absorbance detection enables detection of a wide range of analytes. When exposed to UV light, some compounds also exhibit native fluorescence, which is here detected simultaneously. Calibration curves were measured for serotonin, D-tryptophan and propanolol and separation of the three compounds was performed. All compounds could be detected by both methods, but with a lower limit of detection for native fluorescence.</p>}}, author = {{Ohlsson, P. D. and Ordeig, O. and Mogensen, K. B. and Kutter, J. P.}}, booktitle = {{12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference}}, keywords = {{Absorbance; Electrophoresis; Fluorescence detection; Waveguides}}, language = {{eng}}, pages = {{1618--1620}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Simultaneous native UV fluorescence and absorbance detection for microchip electrophoresis using integrated waveguides}}, year = {{2008}}, }