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Improvement of identification methods for honeybee specific Lactic Acid Bacteria; Future approaches

Lamei, Sepideh LU ; Hu, Yue O O; Olofsson, Tobias C. LU ; Andersson, Anders F; Forsgren, Eva and Vásquez, Alejandra LU (2017) In PLoS ONE 12(3).
Abstract

Honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. The crop microbiota of Apis mellifera, composed of 13 Lactic Acid Bacterial (LAB) species within the genera Lactobacillus and Bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. Possibly playing a vital role in honeybee health, it is important that these honeybee specific Lactic Acid Bacterial (hbs-LAB) symbionts can be correctly identified, isolated and cultured, to further investigate their health promoting properties. We have previously reported successful identification to the strain level by culturedependent methods... (More)

Honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. The crop microbiota of Apis mellifera, composed of 13 Lactic Acid Bacterial (LAB) species within the genera Lactobacillus and Bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. Possibly playing a vital role in honeybee health, it is important that these honeybee specific Lactic Acid Bacterial (hbs-LAB) symbionts can be correctly identified, isolated and cultured, to further investigate their health promoting properties. We have previously reported successful identification to the strain level by culturedependent methods and we recently sequenced and annotated the genomes of the 13 hbs- LAB. However, the hitherto applied techniques are unfortunately very time consuming, expensive and not ideal when analyzing a vast quantity of samples. In addition, other researchers have constantly failed to identify the 13 hbs-LAB from honeybee samples by using inadequate media and/or molecular techniques based on 16S rRNA gene sequencing with insufficient discriminatory power. The aim of this study was to develop better and more suitable methods for the identification and cultivation of hbs-LAB. We compared currently used bacterial cultivation media and could for the first time demonstrate a significant variation in the hbs-LAB basic requirements for optimal growth. We also present a new bacterial identification approach based on amplicon sequencing of a region of the 16S rRNA gene using the Illumina platform and an error correction software that can be used to successfully differentiate and rapidly identify the 13 hbs-LAB to the strain level.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactic Acid Bacteria, honeybee
in
PLoS ONE
volume
12
issue
3
publisher
Public Library of Science
external identifiers
  • scopus:85016432762
  • pmid:28346815
  • wos:000399174300061
ISSN
1932-6203
DOI
10.1371/journal.pone.0174614
language
English
LU publication?
yes
id
3b661c16-9a30-43b1-a074-9684a4480127
date added to LUP
2017-04-24 11:18:15
date last changed
2018-02-28 10:00:07
@article{3b661c16-9a30-43b1-a074-9684a4480127,
  abstract     = {<p>Honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. The crop microbiota of Apis mellifera, composed of 13 Lactic Acid Bacterial (LAB) species within the genera Lactobacillus and Bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. Possibly playing a vital role in honeybee health, it is important that these honeybee specific Lactic Acid Bacterial (hbs-LAB) symbionts can be correctly identified, isolated and cultured, to further investigate their health promoting properties. We have previously reported successful identification to the strain level by culturedependent methods and we recently sequenced and annotated the genomes of the 13 hbs- LAB. However, the hitherto applied techniques are unfortunately very time consuming, expensive and not ideal when analyzing a vast quantity of samples. In addition, other researchers have constantly failed to identify the 13 hbs-LAB from honeybee samples by using inadequate media and/or molecular techniques based on 16S rRNA gene sequencing with insufficient discriminatory power. The aim of this study was to develop better and more suitable methods for the identification and cultivation of hbs-LAB. We compared currently used bacterial cultivation media and could for the first time demonstrate a significant variation in the hbs-LAB basic requirements for optimal growth. We also present a new bacterial identification approach based on amplicon sequencing of a region of the 16S rRNA gene using the Illumina platform and an error correction software that can be used to successfully differentiate and rapidly identify the 13 hbs-LAB to the strain level.</p>},
  articleno    = {e0174614},
  author       = {Lamei, Sepideh and Hu, Yue O O and Olofsson, Tobias C. and Andersson, Anders F and Forsgren, Eva and Vásquez, Alejandra},
  issn         = {1932-6203},
  keyword      = {Lactic Acid Bacteria,honeybee},
  language     = {eng},
  month        = {03},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Improvement of identification methods for honeybee specific Lactic Acid Bacteria; Future approaches},
  url          = {http://dx.doi.org/10.1371/journal.pone.0174614},
  volume       = {12},
  year         = {2017},
}