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Direct assessment of microbial colonisation in damp houses by chemical marker analysis

Szponar, B ; Szponar, A and Larsson, Lennart LU (2003) In Indoor & Built Environment 12(4). p.251-254
Abstract
Ergosterol was used as a marker of fungal biomass and 3-hydroxy fatty acids were used as markers of bacterial endotoxin to determine microbial colonisation of building materials by gas chromatography-mass spectrometry. Pieces (0.3-0.5 g) of materials from two water-damaged buildings were hydrolysed to liberate the markers from larger microbial structures; the preparations were then purified, derivatised, and analysed. The marker patterns obtained revealed that the microbial communities differed between the two buildings both qualitatively and quantitatively. The chemical-analytical method described should be useful for detecting and characterising microbial colonisation on surfaces in, for example, damp buildings.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fungi, bacteria, ergosterol, 3-hydroxy fatty acids, building materials
in
Indoor & Built Environment
volume
12
issue
4
pages
251 - 254
publisher
SAGE Publications
external identifiers
  • wos:000184949300012
  • scopus:0041466165
ISSN
1420-326X
DOI
10.1177/1420326X03035164
language
English
LU publication?
yes
id
3bef6abb-5566-4759-880e-30954df28cea (old id 303530)
date added to LUP
2016-04-01 15:57:45
date last changed
2022-01-28 08:22:22
@article{3bef6abb-5566-4759-880e-30954df28cea,
  abstract     = {{Ergosterol was used as a marker of fungal biomass and 3-hydroxy fatty acids were used as markers of bacterial endotoxin to determine microbial colonisation of building materials by gas chromatography-mass spectrometry. Pieces (0.3-0.5 g) of materials from two water-damaged buildings were hydrolysed to liberate the markers from larger microbial structures; the preparations were then purified, derivatised, and analysed. The marker patterns obtained revealed that the microbial communities differed between the two buildings both qualitatively and quantitatively. The chemical-analytical method described should be useful for detecting and characterising microbial colonisation on surfaces in, for example, damp buildings.}},
  author       = {{Szponar, B and Szponar, A and Larsson, Lennart}},
  issn         = {{1420-326X}},
  keywords     = {{fungi; bacteria; ergosterol; 3-hydroxy fatty acids; building materials}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{251--254}},
  publisher    = {{SAGE Publications}},
  series       = {{Indoor & Built Environment}},
  title        = {{Direct assessment of microbial colonisation in damp houses by chemical marker analysis}},
  url          = {{http://dx.doi.org/10.1177/1420326X03035164}},
  doi          = {{10.1177/1420326X03035164}},
  volume       = {{12}},
  year         = {{2003}},
}