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Binding and elution behavior of small deoxyribonucleic acid fragments on a strong anion-exchanger multimodal chromatography resin.

Matos, Tiago LU ; Queiroz, João A and Bülow, Leif LU (2013) In Journal of Chromatography A 1302. p.40-44
Abstract
The separation behavior of small single-stranded from double-stranded DNA molecules has been determined on a multimodal (mixed-mode) chromatography system. The resin used is a strong anion exchanger which also modulates hydrophobic recognition. The intrinsic differences between single- and double-stranded DNAs concerning charge, hydrophobicity and three-dimensional structure render this form of MMC suitable for separation of the different nucleic acid molecules. All DNAs tested bound strongly to the resin and they could be eluted with increasing NaCl concentrations. Each homopolymeric ssDNA sample resulted in a base-specific elution pattern when using a linear NaCl gradient. The elution order was... (More)
The separation behavior of small single-stranded from double-stranded DNA molecules has been determined on a multimodal (mixed-mode) chromatography system. The resin used is a strong anion exchanger which also modulates hydrophobic recognition. The intrinsic differences between single- and double-stranded DNAs concerning charge, hydrophobicity and three-dimensional structure render this form of MMC suitable for separation of the different nucleic acid molecules. All DNAs tested bound strongly to the resin and they could be eluted with increasing NaCl concentrations. Each homopolymeric ssDNA sample resulted in a base-specific elution pattern when using a linear NaCl gradient. The elution order was poly(dA)<poly(dC)<poly(dG)<poly(dT) and this order was dependent on the secondary structure of the molecule. Such differences were not observed for small double-stranded DNAs. Due to the more hydrophobic nature of single-stranded DNA molecules they could be separated from double-stranded DNAs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1302
pages
40 - 44
publisher
Elsevier
external identifiers
  • wos:000322349700006
  • pmid:23830219
  • scopus:84880278506
ISSN
0021-9673
DOI
10.1016/j.chroma.2013.05.044
language
English
LU publication?
yes
id
3bff4526-655f-4f79-ac2b-c77a181782e1 (old id 3956129)
date added to LUP
2013-08-05 12:51:58
date last changed
2019-02-20 03:16:48
@article{3bff4526-655f-4f79-ac2b-c77a181782e1,
  abstract     = {The separation behavior of small single-stranded from double-stranded DNA molecules has been determined on a multimodal (mixed-mode) chromatography system. The resin used is a strong anion exchanger which also modulates hydrophobic recognition. The intrinsic differences between single- and double-stranded DNAs concerning charge, hydrophobicity and three-dimensional structure render this form of MMC suitable for separation of the different nucleic acid molecules. All DNAs tested bound strongly to the resin and they could be eluted with increasing NaCl concentrations. Each homopolymeric ssDNA sample resulted in a base-specific elution pattern when using a linear NaCl gradient. The elution order was poly(dA)&lt;poly(dC)&lt;poly(dG)&lt;poly(dT) and this order was dependent on the secondary structure of the molecule. Such differences were not observed for small double-stranded DNAs. Due to the more hydrophobic nature of single-stranded DNA molecules they could be separated from double-stranded DNAs.},
  author       = {Matos, Tiago and Queiroz, João A and Bülow, Leif},
  issn         = {0021-9673},
  language     = {eng},
  pages        = {40--44},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Binding and elution behavior of small deoxyribonucleic acid fragments on a strong anion-exchanger multimodal chromatography resin.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2013.05.044},
  volume       = {1302},
  year         = {2013},
}