Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs.
(2011) In Forensic Science International: Genetics 5(3). p.194-198- Abstract
- Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas((R)) Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5-32muL) with high or intermediate amylase activity (840 and 290kU/L). Results were typically obtained within 5min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even... (More)
- Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas((R)) Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5-32muL) with high or intermediate amylase activity (840 and 290kU/L). Results were typically obtained within 5min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even so, a positive amylase result indicates presence of saliva, and thereby presence of DNA. Amylase testing may be useful for screening in investigations where the number of DNA analyses is limited due to cost, e.g., in volume crime. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1610380
- author
- Hedman, Johannes LU ; Dalin, Erik LU ; Rasmusson, Birgitta and Ansell, Ricky
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Forensic Science International: Genetics
- volume
- 5
- issue
- 3
- pages
- 194 - 198
- publisher
- Elsevier
- external identifiers
-
- wos:000289193200019
- pmid:20457099
- scopus:79953897230
- pmid:20457099
- ISSN
- 1878-0326
- DOI
- 10.1016/j.fsigen.2010.03.003
- language
- English
- LU publication?
- yes
- id
- 3c20c5b3-1dcb-488e-95cf-7811821b12f5 (old id 1610380)
- date added to LUP
- 2016-04-01 11:08:38
- date last changed
- 2022-04-12 20:43:51
@article{3c20c5b3-1dcb-488e-95cf-7811821b12f5,
abstract = {{Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas((R)) Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5-32muL) with high or intermediate amylase activity (840 and 290kU/L). Results were typically obtained within 5min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even so, a positive amylase result indicates presence of saliva, and thereby presence of DNA. Amylase testing may be useful for screening in investigations where the number of DNA analyses is limited due to cost, e.g., in volume crime.}},
author = {{Hedman, Johannes and Dalin, Erik and Rasmusson, Birgitta and Ansell, Ricky}},
issn = {{1878-0326}},
language = {{eng}},
number = {{3}},
pages = {{194--198}},
publisher = {{Elsevier}},
series = {{Forensic Science International: Genetics}},
title = {{Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs.}},
url = {{http://dx.doi.org/10.1016/j.fsigen.2010.03.003}},
doi = {{10.1016/j.fsigen.2010.03.003}},
volume = {{5}},
year = {{2011}},
}