Ice coating –A new method of brain device insertion to mitigate acute injuries
(2020) In Journal of Neuroscience Methods 343.- Abstract
Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen... (More)
Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen gelatin embedded needles reduced the insertion force with around 50 %, substantially reduced the loss neurons (i.e. reduced neuronal void), and yielded near normal levels of astrocytes within the insertion track 1 day after insertion, as compared to gelatin coated probes of the same temperature without ice coating. There were negligible effects on glial reactions and neuronal density immediately outside the insertion track of both ice coated and cold gelatin embedded needles. This new method of implantation presents a considerable improvement compared to existing modes of device insertion. Conclusions: Acute brain injuries following insertion of e.g. ultra-flexible electrodes, can be reduced by providing an outer coat of ultra-slippery thawing ice. No adverse effect of lowered implant temperature was found, opening the possibility of locking fragile electrode construct configurations in frozen gelatin, prior to implantation into the brain.
(Less)
- author
- Mohammed, Mohsin
LU
; Thelin, Jonas
LU
; Gällentoft, Lina LU ; Thorbergsson, Palmi Thor LU ; Kumosa, Lucas S. LU ; Schouenborg, Jens LU and Pettersson, Lina M.E. LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biocompatibility, Brain machine interface, Deep brain stimulation, Implantation method, Neurosurgical method
- in
- Journal of Neuroscience Methods
- volume
- 343
- article number
- 108842
- publisher
- Elsevier
- external identifiers
-
- pmid:32628965
- scopus:85087524365
- ISSN
- 0165-0270
- DOI
- 10.1016/j.jneumeth.2020.108842
- language
- English
- LU publication?
- yes
- id
- 3c383524-b6e0-4d8b-a446-fd843dda61d9
- date added to LUP
- 2020-07-14 12:18:49
- date last changed
- 2024-05-29 16:27:21
@article{3c383524-b6e0-4d8b-a446-fd843dda61d9, abstract = {{<p>Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen gelatin embedded needles reduced the insertion force with around 50 %, substantially reduced the loss neurons (i.e. reduced neuronal void), and yielded near normal levels of astrocytes within the insertion track 1 day after insertion, as compared to gelatin coated probes of the same temperature without ice coating. There were negligible effects on glial reactions and neuronal density immediately outside the insertion track of both ice coated and cold gelatin embedded needles. This new method of implantation presents a considerable improvement compared to existing modes of device insertion. Conclusions: Acute brain injuries following insertion of e.g. ultra-flexible electrodes, can be reduced by providing an outer coat of ultra-slippery thawing ice. No adverse effect of lowered implant temperature was found, opening the possibility of locking fragile electrode construct configurations in frozen gelatin, prior to implantation into the brain.</p>}}, author = {{Mohammed, Mohsin and Thelin, Jonas and Gällentoft, Lina and Thorbergsson, Palmi Thor and Kumosa, Lucas S. and Schouenborg, Jens and Pettersson, Lina M.E.}}, issn = {{0165-0270}}, keywords = {{Biocompatibility; Brain machine interface; Deep brain stimulation; Implantation method; Neurosurgical method}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Journal of Neuroscience Methods}}, title = {{Ice coating –A new method of brain device insertion to mitigate acute injuries}}, url = {{http://dx.doi.org/10.1016/j.jneumeth.2020.108842}}, doi = {{10.1016/j.jneumeth.2020.108842}}, volume = {{343}}, year = {{2020}}, }