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Ice coating –A new method of brain device insertion to mitigate acute injuries

Mohammed, Mohsin LU ; Thelin, Jonas LU orcid ; Gällentoft, Lina LU ; Thorbergsson, Palmi Thor LU ; Kumosa, Lucas S. LU ; Schouenborg, Jens LU and Pettersson, Lina M.E. LU (2020) In Journal of Neuroscience Methods 343.
Abstract

Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen... (More)

Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen gelatin embedded needles reduced the insertion force with around 50 %, substantially reduced the loss neurons (i.e. reduced neuronal void), and yielded near normal levels of astrocytes within the insertion track 1 day after insertion, as compared to gelatin coated probes of the same temperature without ice coating. There were negligible effects on glial reactions and neuronal density immediately outside the insertion track of both ice coated and cold gelatin embedded needles. This new method of implantation presents a considerable improvement compared to existing modes of device insertion. Conclusions: Acute brain injuries following insertion of e.g. ultra-flexible electrodes, can be reduced by providing an outer coat of ultra-slippery thawing ice. No adverse effect of lowered implant temperature was found, opening the possibility of locking fragile electrode construct configurations in frozen gelatin, prior to implantation into the brain.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biocompatibility, Brain machine interface, Deep brain stimulation, Implantation method, Neurosurgical method
in
Journal of Neuroscience Methods
volume
343
article number
108842
publisher
Elsevier
external identifiers
  • pmid:32628965
  • scopus:85087524365
ISSN
0165-0270
DOI
10.1016/j.jneumeth.2020.108842
language
English
LU publication?
yes
id
3c383524-b6e0-4d8b-a446-fd843dda61d9
date added to LUP
2020-07-14 12:18:49
date last changed
2024-05-29 16:27:21
@article{3c383524-b6e0-4d8b-a446-fd843dda61d9,
  abstract     = {{<p>Background: Reduction of insertion injury is likely important to approach physiological conditions in the vicinity of implanted devices intended to interface with the surrounding brain. New methods: We have developed a novel, low-friction coating around frozen, gelatin embedded needles. By introducing a layer of thawing ice onto the gelatin, decreasing surface friction, we mitigate damage caused by the implantation. Results and comparison with existing methods: The acute effects of a transient stab on neuronal density and glial reactions were assessed 1 and 7 days post stab in rat cortex and striatum both within and outside the insertion track using immunohistochemical staining. The addition of a coat of melting ice to the frozen gelatin embedded needles reduced the insertion force with around 50 %, substantially reduced the loss neurons (i.e. reduced neuronal void), and yielded near normal levels of astrocytes within the insertion track 1 day after insertion, as compared to gelatin coated probes of the same temperature without ice coating. There were negligible effects on glial reactions and neuronal density immediately outside the insertion track of both ice coated and cold gelatin embedded needles. This new method of implantation presents a considerable improvement compared to existing modes of device insertion. Conclusions: Acute brain injuries following insertion of e.g. ultra-flexible electrodes, can be reduced by providing an outer coat of ultra-slippery thawing ice. No adverse effect of lowered implant temperature was found, opening the possibility of locking fragile electrode construct configurations in frozen gelatin, prior to implantation into the brain.</p>}},
  author       = {{Mohammed, Mohsin and Thelin, Jonas and Gällentoft, Lina and Thorbergsson, Palmi Thor and Kumosa, Lucas S. and Schouenborg, Jens and Pettersson, Lina M.E.}},
  issn         = {{0165-0270}},
  keywords     = {{Biocompatibility; Brain machine interface; Deep brain stimulation; Implantation method; Neurosurgical method}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Neuroscience Methods}},
  title        = {{Ice coating –A new method of brain device insertion to mitigate acute injuries}},
  url          = {{http://dx.doi.org/10.1016/j.jneumeth.2020.108842}},
  doi          = {{10.1016/j.jneumeth.2020.108842}},
  volume       = {{343}},
  year         = {{2020}},
}