Serial digital autoradiography with a silicon strip detector as a high resolution imaging modality for TRT Dosimetry
(2007) IEEE Nuclear Science Symposium/Medical Imaging Conference p.4054-4056- Abstract
- This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step... (More)
- This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step process with least square error and mutual information registration measures. The realigned volumes were convolved with beta dose point kernels to provide the dose rate distribution for I-131 and Y-90 at the time of sacrifice. Using these volumes, comparisons can be made between uptake and penetration of different antibodies and the dose rate uniformity of different radionuclides. Simulations performed using measured I-131 and I-125 energy spectra showed that energy separation with less than 5% error could be performed with 100 counts per pixel. Imaging and subsequent separation of a sample containing both I-131 and I-125 proved the possibility of simultaneous imaging of two targeting agents in the same tissue. Thinner tissue sections were also set aside and successfully used for H&E staining and immunohistochemistry to enable future comparison of uptake and dose rate in different cell-type populations in the tissue. This method successfully provides high-resolution activity and dose rate volumes and has potential for multi-labeling imaging and co-registration with histology. As a complimentary imaging modality it can aid in investigating the effect of non-uniform uptake. Optimization is still needed in both the sectioning protocol and realignment software. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1406779
- author
- Örbom, Anders LU ; Dahlbom, Magnus ; Olafsen, Tove ; Wu, Anna M. and Strand, Sven-Erik LU
- organization
- publishing date
- 2007
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- 2007 IEEE Nuclear Science Symposium Conference Record, vols 1-11
- pages
- 4054 - 4056
- publisher
- IEEE - Institute of Electrical and Electronics Engineers Inc.
- conference name
- IEEE Nuclear Science Symposium/Medical Imaging Conference
- conference location
- Honolulu, HI, United States
- conference dates
- 2007-10-26 - 2007-11-03
- external identifiers
-
- wos:000257380403044
- scopus:48149084791
- ISSN
- 1082-3654
- ISBN
- 978-1-4244-0922-8
- DOI
- 10.1109/NSSMIC.2007.4437018
- language
- English
- LU publication?
- yes
- id
- 3c858cc2-f72b-45c9-bf6a-5a752d17af64 (old id 1406779)
- date added to LUP
- 2016-04-01 16:23:05
- date last changed
- 2022-01-28 19:17:49
@inproceedings{3c858cc2-f72b-45c9-bf6a-5a752d17af64,
abstract = {{This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step process with least square error and mutual information registration measures. The realigned volumes were convolved with beta dose point kernels to provide the dose rate distribution for I-131 and Y-90 at the time of sacrifice. Using these volumes, comparisons can be made between uptake and penetration of different antibodies and the dose rate uniformity of different radionuclides. Simulations performed using measured I-131 and I-125 energy spectra showed that energy separation with less than 5% error could be performed with 100 counts per pixel. Imaging and subsequent separation of a sample containing both I-131 and I-125 proved the possibility of simultaneous imaging of two targeting agents in the same tissue. Thinner tissue sections were also set aside and successfully used for H&E staining and immunohistochemistry to enable future comparison of uptake and dose rate in different cell-type populations in the tissue. This method successfully provides high-resolution activity and dose rate volumes and has potential for multi-labeling imaging and co-registration with histology. As a complimentary imaging modality it can aid in investigating the effect of non-uniform uptake. Optimization is still needed in both the sectioning protocol and realignment software.}},
author = {{Örbom, Anders and Dahlbom, Magnus and Olafsen, Tove and Wu, Anna M. and Strand, Sven-Erik}},
booktitle = {{2007 IEEE Nuclear Science Symposium Conference Record, vols 1-11}},
isbn = {{978-1-4244-0922-8}},
issn = {{1082-3654}},
language = {{eng}},
pages = {{4054--4056}},
publisher = {{IEEE - Institute of Electrical and Electronics Engineers Inc.}},
title = {{Serial digital autoradiography with a silicon strip detector as a high resolution imaging modality for TRT Dosimetry}},
url = {{http://dx.doi.org/10.1109/NSSMIC.2007.4437018}},
doi = {{10.1109/NSSMIC.2007.4437018}},
year = {{2007}},
}