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Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population

Uibo, R ; Sullivan, E P ; Uibo, O ; Lernmark, A LU orcid ; Salur, L ; Kivik, T and Mandel, M (2001) In Hormone and Metabolic Research 33(9). p.7-564
Abstract

We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no... (More)

We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Adolescent, Adult, Aged, Alleles, Autoantibodies/blood, Diabetes Mellitus, Type 1/immunology, Enzyme-Linked Immunosorbent Assay, Estonia, Female, Gliadin/immunology, Glutamate Decarboxylase/immunology, HLA-DR Antigens/genetics, HLA-DRB1 Chains, Humans, Immunoglobulin A/blood, Immunoglobulin G/blood, Islets of Langerhans/immunology, Male, Middle Aged
in
Hormone and Metabolic Research
volume
33
issue
9
pages
7 - 564
publisher
Georg Thieme Verlag
external identifiers
  • scopus:0034808342
  • pmid:11561218
ISSN
0018-5043
DOI
10.1055/s-2001-17215
language
English
LU publication?
no
id
3c97820b-2fb8-4875-b1f4-4db0c20b5a1a
date added to LUP
2021-09-28 16:17:23
date last changed
2024-03-13 08:08:34
@article{3c97820b-2fb8-4875-b1f4-4db0c20b5a1a,
  abstract     = {{<p>We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p &gt; 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p &gt; 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.</p>}},
  author       = {{Uibo, R and Sullivan, E P and Uibo, O and Lernmark, A and Salur, L and Kivik, T and Mandel, M}},
  issn         = {{0018-5043}},
  keywords     = {{Adolescent; Adult; Aged; Alleles; Autoantibodies/blood; Diabetes Mellitus, Type 1/immunology; Enzyme-Linked Immunosorbent Assay; Estonia; Female; Gliadin/immunology; Glutamate Decarboxylase/immunology; HLA-DR Antigens/genetics; HLA-DRB1 Chains; Humans; Immunoglobulin A/blood; Immunoglobulin G/blood; Islets of Langerhans/immunology; Male; Middle Aged}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{7--564}},
  publisher    = {{Georg Thieme Verlag}},
  series       = {{Hormone and Metabolic Research}},
  title        = {{Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population}},
  url          = {{http://dx.doi.org/10.1055/s-2001-17215}},
  doi          = {{10.1055/s-2001-17215}},
  volume       = {{33}},
  year         = {{2001}},
}