Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population
(2001) In Hormone and Metabolic Research 33(9). p.7-564- Abstract
We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no... (More)
We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.
(Less)
- author
- Uibo, R ; Sullivan, E P ; Uibo, O ; Lernmark, A LU ; Salur, L ; Kivik, T and Mandel, M
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- keywords
- Adolescent, Adult, Aged, Alleles, Autoantibodies/blood, Diabetes Mellitus, Type 1/immunology, Enzyme-Linked Immunosorbent Assay, Estonia, Female, Gliadin/immunology, Glutamate Decarboxylase/immunology, HLA-DR Antigens/genetics, HLA-DRB1 Chains, Humans, Immunoglobulin A/blood, Immunoglobulin G/blood, Islets of Langerhans/immunology, Male, Middle Aged
- in
- Hormone and Metabolic Research
- volume
- 33
- issue
- 9
- pages
- 7 - 564
- publisher
- Georg Thieme Verlag
- external identifiers
-
- scopus:0034808342
- pmid:11561218
- ISSN
- 0018-5043
- DOI
- 10.1055/s-2001-17215
- language
- English
- LU publication?
- no
- id
- 3c97820b-2fb8-4875-b1f4-4db0c20b5a1a
- date added to LUP
- 2021-09-28 16:17:23
- date last changed
- 2024-03-13 08:08:34
@article{3c97820b-2fb8-4875-b1f4-4db0c20b5a1a, abstract = {{<p>We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.</p>}}, author = {{Uibo, R and Sullivan, E P and Uibo, O and Lernmark, A and Salur, L and Kivik, T and Mandel, M}}, issn = {{0018-5043}}, keywords = {{Adolescent; Adult; Aged; Alleles; Autoantibodies/blood; Diabetes Mellitus, Type 1/immunology; Enzyme-Linked Immunosorbent Assay; Estonia; Female; Gliadin/immunology; Glutamate Decarboxylase/immunology; HLA-DR Antigens/genetics; HLA-DRB1 Chains; Humans; Immunoglobulin A/blood; Immunoglobulin G/blood; Islets of Langerhans/immunology; Male; Middle Aged}}, language = {{eng}}, number = {{9}}, pages = {{7--564}}, publisher = {{Georg Thieme Verlag}}, series = {{Hormone and Metabolic Research}}, title = {{Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population}}, url = {{http://dx.doi.org/10.1055/s-2001-17215}}, doi = {{10.1055/s-2001-17215}}, volume = {{33}}, year = {{2001}}, }