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Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones

Dircksen, Heinrich; Neupert, Susanne; Predel, Reinhard; Verleyen, Peter; Huybrechts, Jurgen; Strauss, Johannes; Hauser, Frank; Stafflinger, Elisabeth; Schneider, Martina LU and Pauwels, Kevin, et al. (2011) In Journal of Proteome Research 10(10). p.4478-4504
Abstract

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin,... (More)

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

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@article{3ca4ccb4-93a2-4486-953d-3f0abcc5d1a2,
  abstract     = {<p>We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, allatotropin, Ala(7)-CCAP, CCHamide, Arg(7)-corazonin, DENamides, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin, two alternative splice forms of ion transport peptide (ITP), myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met(4)-proctolin, short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, and three tachykinins. There are two genes for a preprohormone containing orcomyotropin-like peptides and orcokinins, two genes for N-terminally elongated ITPs, two genes (clustered) for eclosion hormones, two genes (clustered) for bursicons alpha, beta, and two genes (clustered) for glycoproteins GPA2, GPB5, three genes for different allatostatins-C (two of them clustered) and three genes for IGF-related peptides. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer related to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.</p>},
  author       = {Dircksen, Heinrich and Neupert, Susanne and Predel, Reinhard and Verleyen, Peter and Huybrechts, Jurgen and Strauss, Johannes and Hauser, Frank and Stafflinger, Elisabeth and Schneider, Martina and Pauwels, Kevin and Schoofs, Liliane and Grimmelikhuijzen, Cornelis J P},
  issn         = {1535-3893},
  keyword      = {Adipokines,Amino Acid Sequence,Animals,Computational Biology,Daphnia,Expressed Sequence Tags,Female,Genomics,Mass Spectrometry,Molecular Sequence Data,Neuropeptides,Peptides,Proteins,Proteomics,Reverse Transcriptase Polymerase Chain Reaction,Sequence Homology, Amino Acid,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization,Transcriptome,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {10},
  number       = {10},
  pages        = {4478--4504},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Genomics, transcriptomics, and peptidomics of Daphnia pulex neuropeptides and protein hormones},
  url          = {http://dx.doi.org/10.1021/pr200284e},
  volume       = {10},
  year         = {2011},
}