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Effect of sodium taurodeoxycholate on PEO-PPO-PEO triblock copolymer F127 with incorporated SNAP: Insights into micellization, gelation, and nitric oxide release

Bomediano, Mateus P. LU ; Plivelic, Tomás S. LU ; Pedersen, Jan Skov ; Galantini, Luciano ; Schillén, Karin LU orcid and de Oliveira, Marcelo G. (2026) In Colloids and Surfaces A: Physicochemical and Engineering Aspects 730.
Abstract
We report the first incorporation of S-nitroso-N-acetylpenicillamine (SNAP) into micellar systems of the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer Pluronic® F127 for nitric oxide (NO) delivery and show how sodium taurodeoxycholate (NaTDC) modulates F127 micellization and gelation. Using differential scanning calorimetry, small-angle X-ray scattering, rheology, dynamic light scattering, UV–vis spectroscopy, and chemiluminescence NO analysis, we identify two NaTDC-dependent regimes. At low NaTDC/F127 ratios (MR ≤ ∼1.2), NaTDC associates with F127 micelles, lowering the critical micellization temperature and modestly compacting the PPO cores. At higher ratios (MR ≥ ∼3.8), micellization... (More)
We report the first incorporation of S-nitroso-N-acetylpenicillamine (SNAP) into micellar systems of the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer Pluronic® F127 for nitric oxide (NO) delivery and show how sodium taurodeoxycholate (NaTDC) modulates F127 micellization and gelation. Using differential scanning calorimetry, small-angle X-ray scattering, rheology, dynamic light scattering, UV–vis spectroscopy, and chemiluminescence NO analysis, we identify two NaTDC-dependent regimes. At low NaTDC/F127 ratios (MR ≤ ∼1.2), NaTDC associates with F127 micelles, lowering the critical micellization temperature and modestly compacting the PPO cores. At higher ratios (MR ≥ ∼3.8), micellization becomes less cooperative, small bile salt–rich complexes form, and at 25 wt% F127, the gel nanostructure transitions from face-centered cubic phase to body-centered cubic phase and eventually loses long-range order (MR ∼6.2). SNAP load does not alter micellization, locates at the core–corona interface, and shows enhanced solubility that depends more on F127 concentration than on NaTDC. Thermal NO release from SNAP (25 °C, dark) follows second-order kinetics, slowing as F127 concentration increases (1–25 wt%), resulting in prolonged NO release. These findings contribute to advancing the understanding of the behavior of PEO-PPO-PEO/NaTDC micellar systems, by demonstrating how NaTDC regulates micellization and gelation. Moreover, the finding that F127 concentration controls SNAP stability and NO-release kinetics, makes the F127/NaTDC system a potential modular platform for sustained and localized NO delivery. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces A: Physicochemical and Engineering Aspects
volume
730
article number
138969
pages
15 pages
publisher
Elsevier
ISSN
0927-7757
DOI
10.1016/j.colsurfa.2025.138969
language
English
LU publication?
yes
id
3cc9e458-2195-4600-8028-04640ee4858b
date added to LUP
2025-11-23 18:29:28
date last changed
2025-11-24 10:36:04
@article{3cc9e458-2195-4600-8028-04640ee4858b,
  abstract     = {{We report the first incorporation of S-nitroso-N-acetylpenicillamine (SNAP) into micellar systems of the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer Pluronic® F127 for nitric oxide (NO) delivery and show how sodium taurodeoxycholate (NaTDC) modulates F127 micellization and gelation. Using differential scanning calorimetry, small-angle X-ray scattering, rheology, dynamic light scattering, UV–vis spectroscopy, and chemiluminescence NO analysis, we identify two NaTDC-dependent regimes. At low NaTDC/F127 ratios (MR ≤ ∼1.2), NaTDC associates with F127 micelles, lowering the critical micellization temperature and modestly compacting the PPO cores. At higher ratios (MR ≥ ∼3.8), micellization becomes less cooperative, small bile salt–rich complexes form, and at 25 wt% F127, the gel nanostructure transitions from face-centered cubic phase to body-centered cubic phase and eventually loses long-range order (MR ∼6.2). SNAP load does not alter micellization, locates at the core–corona interface, and shows enhanced solubility that depends more on F127 concentration than on NaTDC. Thermal NO release from SNAP (25 °C, dark) follows second-order kinetics, slowing as F127 concentration increases (1–25 wt%), resulting in prolonged NO release. These findings contribute to advancing the understanding of the behavior of PEO-PPO-PEO/NaTDC micellar systems, by demonstrating how NaTDC regulates micellization and gelation. Moreover, the finding that F127 concentration controls SNAP stability and NO-release kinetics, makes the F127/NaTDC system a potential modular platform for sustained and localized NO delivery.}},
  author       = {{Bomediano, Mateus P. and Plivelic, Tomás S. and Pedersen, Jan Skov and Galantini, Luciano and Schillén, Karin and de Oliveira, Marcelo G.}},
  issn         = {{0927-7757}},
  language     = {{eng}},
  month        = {{02}},
  publisher    = {{Elsevier}},
  series       = {{Colloids and Surfaces A: Physicochemical and Engineering Aspects}},
  title        = {{Effect of sodium taurodeoxycholate on PEO-PPO-PEO triblock copolymer F127 with incorporated SNAP: Insights into micellization, gelation, and nitric oxide release}},
  url          = {{http://dx.doi.org/10.1016/j.colsurfa.2025.138969}},
  doi          = {{10.1016/j.colsurfa.2025.138969}},
  volume       = {{730}},
  year         = {{2026}},
}