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Feedback architectures to regulate flux of components in artificial gene networks

Giordano, Giulia LU ; Franco, Elisa and Murray, Richard M. (2013) American Control Conference, 2013 In 2013 American Control Conference (ACC 2013) p.4747-4752
Abstract

This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative... (More)

This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative feedback architecture is also numerically studied.

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Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
in
2013 American Control Conference (ACC 2013)
pages
6 pages
conference name
American Control Conference, 2013
external identifiers
  • scopus:84883503878
ISBN
9781479901777
DOI
10.1109/ACC.2013.6580572
language
English
LU publication?
no
id
3d21f71d-6640-41d5-bbea-eea9efc7a58c
date added to LUP
2016-07-06 15:29:02
date last changed
2017-02-12 04:34:10
@inproceedings{3d21f71d-6640-41d5-bbea-eea9efc7a58c,
  abstract     = {<p>This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative feedback architecture is also numerically studied.</p>},
  author       = {Giordano, Giulia and Franco, Elisa and Murray, Richard M.},
  booktitle    = {2013 American Control Conference (ACC 2013) },
  isbn         = {9781479901777},
  language     = {eng},
  pages        = {4747--4752},
  title        = {Feedback architectures to regulate flux of components in artificial gene networks},
  url          = {http://dx.doi.org/10.1109/ACC.2013.6580572},
  year         = {2013},
}