Feedback architectures to regulate flux of components in artificial gene networks
(2013) American Control Conference, 2013 p.4747-4752- Abstract
This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative... (More)
This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative feedback architecture is also numerically studied.
(Less)
- author
- Giordano, Giulia LU ; Franco, Elisa and Murray, Richard M.
- organization
- publishing date
- 2013
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- host publication
- 2013 American Control Conference (ACC 2013)
- article number
- 6580572
- pages
- 6 pages
- conference name
- American Control Conference, 2013
- conference location
- Washington, DC, United States
- conference dates
- 2013-06-17 - 2016-06-19
- external identifiers
-
- scopus:84883503878
- ISBN
- 9781479901777
- DOI
- 10.1109/ACC.2013.6580572
- language
- English
- LU publication?
- no
- id
- 3d21f71d-6640-41d5-bbea-eea9efc7a58c
- date added to LUP
- 2016-07-06 15:29:02
- date last changed
- 2022-04-01 01:12:16
@inproceedings{3d21f71d-6640-41d5-bbea-eea9efc7a58c, abstract = {{<p>This paper focuses on the regulation of RNA production for in vitro synthetic gene networks. We consider the problem of matching the production rate of several RNA species concurrently transcribed to form RNA complexes. We design regulatory feedback loops based on two schemes: negative auto-regulation (which can minimize the potentially harmful amount of molecules not used to form products) and cross-activation (which can maximize the overall output flux). We analyze numerically the performance of these schemes: in both cases, transcription rate matching can be achieved through proper feedback constants; negative feedback is faster and maintains stability. A possible experimental implementation of a three and four genes negative feedback architecture is also numerically studied.</p>}}, author = {{Giordano, Giulia and Franco, Elisa and Murray, Richard M.}}, booktitle = {{2013 American Control Conference (ACC 2013)}}, isbn = {{9781479901777}}, language = {{eng}}, pages = {{4747--4752}}, title = {{Feedback architectures to regulate flux of components in artificial gene networks}}, url = {{http://dx.doi.org/10.1109/ACC.2013.6580572}}, doi = {{10.1109/ACC.2013.6580572}}, year = {{2013}}, }