Unique epitopes of glutamic acid decarboxylase autoantibodies in slowly progressive type 1 diabetes.
Kobayashi, Tetsuro ; Tanaka, Shoichiro ; Okubo, Minoru ; Nakanishi, Koji ; Murase, Toshio and Lernmark, Åke LU
(2003)
In Journal of Clinical Endocrinology and Metabolism
88(10).
p.4768-4775
- Abstract
- Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in... (More)
- Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in SPIDDM inversely correlated with the period in which insulin was not required. GAD65Ab in AIDDM did not react with N-terminal epitope located between amino acids 1-83, irrespective of the titer of GAD65Ab. A novel epitope of GAD65Ab in AIDDM residing (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127396
- author
- Kobayashi, Tetsuro
; Tanaka, Shoichiro
; Okubo, Minoru
; Nakanishi, Koji
; Murase, Toshio
and Lernmark, Åke
LU
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Endocrinology and Metabolism
- volume
- 88
- issue
- 10
- pages
- 4768 - 4775
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0242351804
- ISSN
- 1945-7197
- DOI
- 10.1210/jc.2002-021529
- language
- English
- LU publication?
- no
- id
- 3d2ac1be-60fe-45ed-8534-0674453d9cd3 (old id 1127396)
- date added to LUP
- 2016-04-01 15:27:11
- date last changed
- 2022-01-28 05:27:04
@article{3d2ac1be-60fe-45ed-8534-0674453d9cd3,
abstract = {{Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in SPIDDM inversely correlated with the period in which insulin was not required. GAD65Ab in AIDDM did not react with N-terminal epitope located between amino acids 1-83, irrespective of the titer of GAD65Ab. A novel epitope of GAD65Ab in AIDDM residing}},
author = {{Kobayashi, Tetsuro and Tanaka, Shoichiro and Okubo, Minoru and Nakanishi, Koji and Murase, Toshio and Lernmark, Åke}},
issn = {{1945-7197}},
language = {{eng}},
number = {{10}},
pages = {{4768--4775}},
publisher = {{Oxford University Press}},
series = {{Journal of Clinical Endocrinology and Metabolism}},
title = {{Unique epitopes of glutamic acid decarboxylase autoantibodies in slowly progressive type 1 diabetes.}},
url = {{http://dx.doi.org/10.1210/jc.2002-021529}},
doi = {{10.1210/jc.2002-021529}},
volume = {{88}},
year = {{2003}},
}