Calpain activation and apoptosis in motor neurons of cultured adult mouse spinal cord
(2007) In Functional Neurology 22(2). p.105-110- Abstract
- Calpain, a Ca2+-dependent cysteine protease, has been implicated in neuronal apoptosis following spinal cord injury. In this study, activation of calpain was investigated in motor neurons of adult spinal cord slices from the mouse, using a cell-permeable fluorogenic calpain substrate and Western blotting. Calpain was rapidly activated in the motor neurons of excised spinal cord slices and calpain activity was observed both in the cytoplasm and the nuclei. In these neurons, nuclear and chromatin condensation were pronounced. Both calpain inhibitor VI and EGTA (ethyleneglycol-bis(beta-aminoethyl ether) N',N',N',N'-tetraacetic acid) inhibited calpain activation and subsequent appearance of apoptotic nuclei. In contrast, the general caspase... (More)
- Calpain, a Ca2+-dependent cysteine protease, has been implicated in neuronal apoptosis following spinal cord injury. In this study, activation of calpain was investigated in motor neurons of adult spinal cord slices from the mouse, using a cell-permeable fluorogenic calpain substrate and Western blotting. Calpain was rapidly activated in the motor neurons of excised spinal cord slices and calpain activity was observed both in the cytoplasm and the nuclei. In these neurons, nuclear and chromatin condensation were pronounced. Both calpain inhibitor VI and EGTA (ethyleneglycol-bis(beta-aminoethyl ether) N',N',N',N'-tetraacetic acid) inhibited calpain activation and subsequent appearance of apoptotic nuclei. In contrast, the general caspase inhibitor Z-VAD.fmk had no effect. Calpain activation was also observed in the slices by Western blotting using an antibody to 150-kD calpain-cleaved a-fodrin fragment. These results show that calpain is rapidly activated in injured motor neurons and imply that this activation could be responsible for execution of caspase-independent apoptosis in injured adult motor neurons. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/691953
- author
- Momeni, Hamid Reza ; Azadi, Seifollah LU and Kanje, Martin LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- organ culture, motor neurons, caspase, apoptosis, calpain, spinal cord
- in
- Functional Neurology
- volume
- 22
- issue
- 2
- pages
- 105 - 110
- publisher
- CIC Edizioni Internazionali
- external identifiers
-
- wos:000248938700007
- scopus:34547918820
- ISSN
- 0393-5264
- language
- English
- LU publication?
- yes
- id
- 3d69219b-b0fc-4735-8921-87d4d2bb1892 (old id 691953)
- alternative location
- http://www.functionalneurology.it/index.php?PAGE=archivio
- date added to LUP
- 2016-04-01 16:06:59
- date last changed
- 2022-01-28 17:22:48
@article{3d69219b-b0fc-4735-8921-87d4d2bb1892,
abstract = {{Calpain, a Ca2+-dependent cysteine protease, has been implicated in neuronal apoptosis following spinal cord injury. In this study, activation of calpain was investigated in motor neurons of adult spinal cord slices from the mouse, using a cell-permeable fluorogenic calpain substrate and Western blotting. Calpain was rapidly activated in the motor neurons of excised spinal cord slices and calpain activity was observed both in the cytoplasm and the nuclei. In these neurons, nuclear and chromatin condensation were pronounced. Both calpain inhibitor VI and EGTA (ethyleneglycol-bis(beta-aminoethyl ether) N',N',N',N'-tetraacetic acid) inhibited calpain activation and subsequent appearance of apoptotic nuclei. In contrast, the general caspase inhibitor Z-VAD.fmk had no effect. Calpain activation was also observed in the slices by Western blotting using an antibody to 150-kD calpain-cleaved a-fodrin fragment. These results show that calpain is rapidly activated in injured motor neurons and imply that this activation could be responsible for execution of caspase-independent apoptosis in injured adult motor neurons.}},
author = {{Momeni, Hamid Reza and Azadi, Seifollah and Kanje, Martin}},
issn = {{0393-5264}},
keywords = {{organ culture; motor neurons; caspase; apoptosis; calpain; spinal cord}},
language = {{eng}},
number = {{2}},
pages = {{105--110}},
publisher = {{CIC Edizioni Internazionali}},
series = {{Functional Neurology}},
title = {{Calpain activation and apoptosis in motor neurons of cultured adult mouse spinal cord}},
url = {{http://www.functionalneurology.it/index.php?PAGE=archivio}},
volume = {{22}},
year = {{2007}},
}