HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.
(2013) In Methods in Molecular Biology 919. p.297-307- Abstract
- The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3123960
- author
- Lavant, Ewa H and Carlson, Joyce LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 919
- pages
- 297 - 307
- publisher
- Springer
- external identifiers
-
- pmid:22976110
- pmid:22976110
- scopus:84934439112
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-62703-029-8_26
- language
- English
- LU publication?
- yes
- id
- 3e0b0aa7-0273-4afc-b081-b5401fe0d963 (old id 3123960)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22976110?dopt=Abstract
- date added to LUP
- 2016-04-04 07:41:51
- date last changed
- 2022-01-29 02:27:28
@article{3e0b0aa7-0273-4afc-b081-b5401fe0d963, abstract = {{The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.}}, author = {{Lavant, Ewa H and Carlson, Joyce}}, issn = {{1940-6029}}, language = {{eng}}, pages = {{297--307}}, publisher = {{Springer}}, series = {{Methods in Molecular Biology}}, title = {{HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.}}, url = {{http://dx.doi.org/10.1007/978-1-62703-029-8_26}}, doi = {{10.1007/978-1-62703-029-8_26}}, volume = {{919}}, year = {{2013}}, }