Protein separation using metal ion-bound particles in aqueous two-phase system
(1999) In Biotechnology Techniques 13(2). p.145-148- Abstract
Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.
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https://lup.lub.lu.se/record/3e0faffe-e006-4e45-a5e3-ee2406a0638d
- author
- Kwon, Yun Joong and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 1999-04-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Affinity partitioning, Aqueous two-phase system, Lysozyme, Protein separation
- in
- Biotechnology Techniques
- volume
- 13
- issue
- 2
- pages
- 4 pages
- publisher
- Springer
- external identifiers
-
- scopus:0033035506
- ISSN
- 0951-208X
- DOI
- 10.1023/A:1008943624956
- language
- English
- LU publication?
- yes
- id
- 3e0faffe-e006-4e45-a5e3-ee2406a0638d
- date added to LUP
- 2019-07-12 04:38:05
- date last changed
- 2022-01-31 23:27:26
@article{3e0faffe-e006-4e45-a5e3-ee2406a0638d, abstract = {{<p>Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.</p>}}, author = {{Kwon, Yun Joong and Hatti-Kaul, Rajni}}, issn = {{0951-208X}}, keywords = {{Affinity partitioning; Aqueous two-phase system; Lysozyme; Protein separation}}, language = {{eng}}, month = {{04}}, number = {{2}}, pages = {{145--148}}, publisher = {{Springer}}, series = {{Biotechnology Techniques}}, title = {{Protein separation using metal ion-bound particles in aqueous two-phase system}}, url = {{http://dx.doi.org/10.1023/A:1008943624956}}, doi = {{10.1023/A:1008943624956}}, volume = {{13}}, year = {{1999}}, }