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Protein separation using metal ion-bound particles in aqueous two-phase system

Kwon, Yun Joong and Hatti-Kaul, Rajni LU (1999) In Biotechnology Techniques 13(2). p.145-148
Abstract

Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.

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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Affinity partitioning, Aqueous two-phase system, Lysozyme, Protein separation
in
Biotechnology Techniques
volume
13
issue
2
pages
4 pages
publisher
Springer
external identifiers
  • scopus:0033035506
ISSN
0951-208X
DOI
10.1023/A:1008943624956
language
English
LU publication?
yes
id
3e0faffe-e006-4e45-a5e3-ee2406a0638d
date added to LUP
2019-07-12 04:38:05
date last changed
2022-01-31 23:27:26
@article{3e0faffe-e006-4e45-a5e3-ee2406a0638d,
  abstract     = {{<p>Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.</p>}},
  author       = {{Kwon, Yun Joong and Hatti-Kaul, Rajni}},
  issn         = {{0951-208X}},
  keywords     = {{Affinity partitioning; Aqueous two-phase system; Lysozyme; Protein separation}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{2}},
  pages        = {{145--148}},
  publisher    = {{Springer}},
  series       = {{Biotechnology Techniques}},
  title        = {{Protein separation using metal ion-bound particles in aqueous two-phase system}},
  url          = {{http://dx.doi.org/10.1023/A:1008943624956}},
  doi          = {{10.1023/A:1008943624956}},
  volume       = {{13}},
  year         = {{1999}},
}