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Evaluation of perfluorohexyloctane/polydimethylsiloxane for pancreas preservation for clinical islet isolation and transplantation

Ståhle, Magnus; Foss, Aksel; Gustafsson, Bengt LU ; Lempinen, Marko; Lundgren, Torbjörn; Rafael, Ehab LU ; Tufveson, Gunnar; Theisinger, Bastian; Brandhorst, Daniel and Korsgren, Olle, et al. (2016) In Cell Transplantation 25(12). p.2269-2276
Abstract

This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) <10 h and 2) an extended CIT >20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard preservation solutions, that is, University of Wisconsin (UW) or Custodiol. F6H5S5 was preoxygenated for at least 15 min. Included clinical-grade pancreases were procured in UW or Custodiol. Upon arrival at the islet isolation laboratory, the duodenum was removed followed by rough trimming while F6H8S5 was oxygenated for 15–20 min. Trimmed pancreases were immersed into oxygenated F6H8S5 and stored at 4°C... (More)

This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) <10 h and 2) an extended CIT >20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard preservation solutions, that is, University of Wisconsin (UW) or Custodiol. F6H5S5 was preoxygenated for at least 15 min. Included clinical-grade pancreases were procured in UW or Custodiol. Upon arrival at the islet isolation laboratory, the duodenum was removed followed by rough trimming while F6H8S5 was oxygenated for 15–20 min. Trimmed pancreases were immersed into oxygenated F6H8S5 and stored at 4°C overnight followed by subsequent islet isolation. Pancreas preservation using F6H8S5 proved as effective as UW and Custadiol when used within CIT up to 10 h, in terms of both isolation outcome and islet functionality. Preservation in F6H8S5 of pancreases with extended CIT gave results similar to controls with CIT <10 h for both isolated islet functionality and isolation outcome. This study of clinically obtained pancreases indicates a clear benefit of using F6H8S5 on pancreases with extended CIT as it seems to allow extended cold ischemic time without affecting islet function and islet numbers.

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publication status
published
subject
keywords
Clinical islet transplantation, Hypoxia, Islet isolation, Oxygenation, Pancreas preservation, Perfluorohydrocarbons
in
Cell Transplantation
volume
25
issue
12
pages
8 pages
publisher
Cognizant Communication Corporation
external identifiers
  • scopus:85007035461
  • wos:000390183200014
ISSN
0963-6897
DOI
10.3727/096368916X691709
language
English
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yes
id
3e2fc477-4b4a-41e4-963e-9417df560282
date added to LUP
2017-01-20 14:53:51
date last changed
2017-09-18 11:32:58
@article{3e2fc477-4b4a-41e4-963e-9417df560282,
  abstract     = {<p>This study aimed to evaluate a 50:50 mix of perfluorohexyloctane/polydimethylsiloxane 5 (F6H8S5) preservation of pancreases in a clinical setting compared with standard solutions for 1) cold ischemia time (CIT) &lt;10 h and 2) an extended CIT &gt;20 h. Procured clinical-grade pancreases were shipped in either F6H8S5 or in standard preservation solutions, that is, University of Wisconsin (UW) or Custodiol. F6H5S5 was preoxygenated for at least 15 min. Included clinical-grade pancreases were procured in UW or Custodiol. Upon arrival at the islet isolation laboratory, the duodenum was removed followed by rough trimming while F6H8S5 was oxygenated for 15–20 min. Trimmed pancreases were immersed into oxygenated F6H8S5 and stored at 4°C overnight followed by subsequent islet isolation. Pancreas preservation using F6H8S5 proved as effective as UW and Custadiol when used within CIT up to 10 h, in terms of both isolation outcome and islet functionality. Preservation in F6H8S5 of pancreases with extended CIT gave results similar to controls with CIT &lt;10 h for both isolated islet functionality and isolation outcome. This study of clinically obtained pancreases indicates a clear benefit of using F6H8S5 on pancreases with extended CIT as it seems to allow extended cold ischemic time without affecting islet function and islet numbers.</p>},
  author       = {Ståhle, Magnus and Foss, Aksel and Gustafsson, Bengt and Lempinen, Marko and Lundgren, Torbjörn and Rafael, Ehab and Tufveson, Gunnar and Theisinger, Bastian and Brandhorst, Daniel and Korsgren, Olle and Friberg, Andrew},
  issn         = {0963-6897},
  keyword      = {Clinical islet transplantation,Hypoxia,Islet isolation,Oxygenation,Pancreas preservation,Perfluorohydrocarbons},
  language     = {eng},
  number       = {12},
  pages        = {2269--2276},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Evaluation of perfluorohexyloctane/polydimethylsiloxane for pancreas preservation for clinical islet isolation and transplantation},
  url          = {http://dx.doi.org/10.3727/096368916X691709},
  volume       = {25},
  year         = {2016},
}