Clinical-scale generation of strongly CD83-expressing dendritic cells using extracorporeal photopheresis
(2007) In Photodermatology, Photoimmunology & Photomedicine 23(4). p.113-119- Abstract
- Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth... (More)
- Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi-colour flow cytometry. Results: We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression. Conclusions: With a procedure appropriate for routine clinical use, a total number of 10(6)-10(7) DC ready for patient reinfusion can be prepared within 24 h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti-tumour defence reaction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/647654
- author
- Bredberg, Anders LU ; Jonsson, Svante LU ; Lindblom, Anders and Björk, Peter LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cancer immunotherapy, extracorporeal, dendritic cells, CD83, photopheresis, monocytes, clinical scale
- in
- Photodermatology, Photoimmunology & Photomedicine
- volume
- 23
- issue
- 4
- pages
- 113 - 119
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000247583000002
- scopus:34447121562
- pmid:17598863
- ISSN
- 1600-0781
- DOI
- 10.1111/j.1600-0781.2007.00285.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200), Clinical Microbiology, Malmö (013011000)
- id
- 3ead789e-d28f-47d0-a60a-ce65ceed7f73 (old id 647654)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17598863&dopt=Abstract
- date added to LUP
- 2016-04-01 11:47:32
- date last changed
- 2022-01-26 18:18:58
@article{3ead789e-d28f-47d0-a60a-ce65ceed7f73,
abstract = {{Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi-colour flow cytometry. Results: We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression. Conclusions: With a procedure appropriate for routine clinical use, a total number of 10(6)-10(7) DC ready for patient reinfusion can be prepared within 24 h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti-tumour defence reaction.}},
author = {{Bredberg, Anders and Jonsson, Svante and Lindblom, Anders and Björk, Peter}},
issn = {{1600-0781}},
keywords = {{cancer immunotherapy; extracorporeal; dendritic cells; CD83; photopheresis; monocytes; clinical scale}},
language = {{eng}},
number = {{4}},
pages = {{113--119}},
publisher = {{Wiley-Blackwell}},
series = {{Photodermatology, Photoimmunology & Photomedicine}},
title = {{Clinical-scale generation of strongly CD83-expressing dendritic cells using extracorporeal photopheresis}},
url = {{http://dx.doi.org/10.1111/j.1600-0781.2007.00285.x}},
doi = {{10.1111/j.1600-0781.2007.00285.x}},
volume = {{23}},
year = {{2007}},
}