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Developmental and tissue-specific expression of alpha 1-microglobulin mRNA in the rat

Kastern, W ; Björck, L LU and Åkerström, B LU (1986) In Journal of Biological Chemistry 261(32). p.4-15070
Abstract

A rat liver cDNA library was constructed in the lambda gt11 expression vector. Three clones expressing alpha 1-microglobulin, an immunosuppressive plasma protein, were detected by screening with rabbit antiserum against rat alpha 1-microglobulin. The alpha 1-microglobulin activity from one of the clones, 6b, was confirmed with monoclonal antibodies in a solid phase radioimmunoassay. The nucleotide sequence of the fragment (165 base pairs) was determined, and the translated amino acid sequence (55 amino acids) showed a 75% homology to human alpha 1-microglobulin (position 122-176). Southern blots of restriction endonuclease-digested rat DNA indicated two distinct genes with alpha 1-microglobulin homology when probed with radioactive cDNA... (More)

A rat liver cDNA library was constructed in the lambda gt11 expression vector. Three clones expressing alpha 1-microglobulin, an immunosuppressive plasma protein, were detected by screening with rabbit antiserum against rat alpha 1-microglobulin. The alpha 1-microglobulin activity from one of the clones, 6b, was confirmed with monoclonal antibodies in a solid phase radioimmunoassay. The nucleotide sequence of the fragment (165 base pairs) was determined, and the translated amino acid sequence (55 amino acids) showed a 75% homology to human alpha 1-microglobulin (position 122-176). Southern blots of restriction endonuclease-digested rat DNA indicated two distinct genes with alpha 1-microglobulin homology when probed with radioactive cDNA fragment from clone 6b. Northern blots showed the presence of a single mRNA species in rat liver, and the level was low in 1-month-old animals, increased to reach a maximum during adulthood (3 months), and decreased with aging (12 months). The alpha 1-microglobulin concentration in rat serum showed the same age dependence between 1 and 12 months, with the highest values at 3 months. Embryonic development (8.5-day to 17.5-day) was studied using total fetal RNA, and expression of alpha 1-microglobulin mRNA was detected in low amounts only at day 15.5. alpha 1-Microglobulin mRNA levels, studied by an RNA dot blot assay, were high in liver and kidney, low in brain and testis, and none were found in hypothalamus and spleen cells.

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organization
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type
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publication status
published
subject
keywords
Aging, Alpha-Globulins/genetics, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA/metabolism, Fetus, Humans, Liver/growth & development, Male, RNA, Messenger/genetics, Rats, Rats, Inbred Strains, Tissue Distribution
in
Journal of Biological Chemistry
volume
261
issue
32
pages
4 - 15070
publisher
ASBMB
external identifiers
  • scopus:0022980816
  • pmid:2429963
ISSN
0021-9258
language
English
LU publication?
yes
id
3f227104-f559-4925-82f7-bed5fb04993d
alternative location
http://www.jbc.org/content/261/32/15070.long
date added to LUP
2019-05-22 10:32:55
date last changed
2020-01-13 01:52:00
@article{3f227104-f559-4925-82f7-bed5fb04993d,
  abstract     = {<p>A rat liver cDNA library was constructed in the lambda gt11 expression vector. Three clones expressing alpha 1-microglobulin, an immunosuppressive plasma protein, were detected by screening with rabbit antiserum against rat alpha 1-microglobulin. The alpha 1-microglobulin activity from one of the clones, 6b, was confirmed with monoclonal antibodies in a solid phase radioimmunoassay. The nucleotide sequence of the fragment (165 base pairs) was determined, and the translated amino acid sequence (55 amino acids) showed a 75% homology to human alpha 1-microglobulin (position 122-176). Southern blots of restriction endonuclease-digested rat DNA indicated two distinct genes with alpha 1-microglobulin homology when probed with radioactive cDNA fragment from clone 6b. Northern blots showed the presence of a single mRNA species in rat liver, and the level was low in 1-month-old animals, increased to reach a maximum during adulthood (3 months), and decreased with aging (12 months). The alpha 1-microglobulin concentration in rat serum showed the same age dependence between 1 and 12 months, with the highest values at 3 months. Embryonic development (8.5-day to 17.5-day) was studied using total fetal RNA, and expression of alpha 1-microglobulin mRNA was detected in low amounts only at day 15.5. alpha 1-Microglobulin mRNA levels, studied by an RNA dot blot assay, were high in liver and kidney, low in brain and testis, and none were found in hypothalamus and spleen cells.</p>},
  author       = {Kastern, W and Björck, L and Åkerström, B},
  issn         = {0021-9258},
  language     = {eng},
  month        = {11},
  number       = {32},
  pages        = {4--15070},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Developmental and tissue-specific expression of alpha 1-microglobulin mRNA in the rat},
  url          = {http://www.jbc.org/content/261/32/15070.long},
  volume       = {261},
  year         = {1986},
}