Food-borne outbreak of gastroenteritis associated with genogroup I calicivirus.
(2002) In Journal of Clinical Microbiology 40(3). p.794-798- Abstract
- An outbreak of gastroenteritis affecting 158 of 219 (72%) guests and employees at a hotel is described. Food served at the hotel restaurant is believed to have been the source of the outbreak and to have been contaminated by sick employees working in the restaurant. A secondary attack rate of 22% was seen involving 43 persons in all. In stool specimens from seven of eight patients, Norwalk-like viruses (NLVs) were detected by electron microscopy. While NLV-specific PCR using primers JV12 and JV13 were negative, all specimens examined with primers NVp69 and NVp110 were positive. The failure of primers JV12 and JV13 was attributed to several mismatches in the JV12 primer. Genotyping and sequence analysis revealed that all samples had... (More)
- An outbreak of gastroenteritis affecting 158 of 219 (72%) guests and employees at a hotel is described. Food served at the hotel restaurant is believed to have been the source of the outbreak and to have been contaminated by sick employees working in the restaurant. A secondary attack rate of 22% was seen involving 43 persons in all. In stool specimens from seven of eight patients, Norwalk-like viruses (NLVs) were detected by electron microscopy. While NLV-specific PCR using primers JV12 and JV13 were negative, all specimens examined with primers NVp69 and NVp110 were positive. The failure of primers JV12 and JV13 was attributed to several mismatches in the JV12 primer. Genotyping and sequence analysis revealed that all samples had identical sequences and clustered with genogroup I, and the most closely related well-characterized genotype is Desert Shield. This is the first described food-borne outbreak associated with genogroup I virus in Sweden. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106268
- author
- Johansson, Hugo LU ; Torvén, Maria ; Hammarlund, Ann-Christin ; Björne, Ulla ; Hedlund, Kjell-Olof and Svensson, Lennart
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Caliciviridae Infections/*epidemiology/virology, Child, Preschool, *Disease Outbreaks, Feces/virology, Food Poisoning/*epidemiology/virology, Gastroenteritis/*epidemiology/virology, Genotype, Human, Incidence, Middle Age, Non-U.S. Gov't, Support, 80 and over, Caliciviridae/*classification, Aged, Adult, Adolescence
- in
- Journal of Clinical Microbiology
- volume
- 40
- issue
- 3
- pages
- 794 - 798
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:11880395
- wos:000174238600010
- scopus:0036197325
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.40.3.794-798.2002
- language
- English
- LU publication?
- yes
- id
- 3f395a7c-1c4f-4900-bb5a-535c16036bf2 (old id 106268)
- date added to LUP
- 2016-04-01 17:07:30
- date last changed
- 2022-01-29 00:33:46
@article{3f395a7c-1c4f-4900-bb5a-535c16036bf2, abstract = {{An outbreak of gastroenteritis affecting 158 of 219 (72%) guests and employees at a hotel is described. Food served at the hotel restaurant is believed to have been the source of the outbreak and to have been contaminated by sick employees working in the restaurant. A secondary attack rate of 22% was seen involving 43 persons in all. In stool specimens from seven of eight patients, Norwalk-like viruses (NLVs) were detected by electron microscopy. While NLV-specific PCR using primers JV12 and JV13 were negative, all specimens examined with primers NVp69 and NVp110 were positive. The failure of primers JV12 and JV13 was attributed to several mismatches in the JV12 primer. Genotyping and sequence analysis revealed that all samples had identical sequences and clustered with genogroup I, and the most closely related well-characterized genotype is Desert Shield. This is the first described food-borne outbreak associated with genogroup I virus in Sweden.}}, author = {{Johansson, Hugo and Torvén, Maria and Hammarlund, Ann-Christin and Björne, Ulla and Hedlund, Kjell-Olof and Svensson, Lennart}}, issn = {{1098-660X}}, keywords = {{Caliciviridae Infections/*epidemiology/virology; Child; Preschool; *Disease Outbreaks; Feces/virology; Food Poisoning/*epidemiology/virology; Gastroenteritis/*epidemiology/virology; Genotype; Human; Incidence; Middle Age; Non-U.S. Gov't; Support; 80 and over; Caliciviridae/*classification; Aged; Adult; Adolescence}}, language = {{eng}}, number = {{3}}, pages = {{794--798}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Clinical Microbiology}}, title = {{Food-borne outbreak of gastroenteritis associated with genogroup I calicivirus.}}, url = {{https://lup.lub.lu.se/search/files/4881930/623573.pdf}}, doi = {{10.1128/JCM.40.3.794-798.2002}}, volume = {{40}}, year = {{2002}}, }