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Bacterial community tolerance to Cu in soils with geochemical baseline concentrations (GBCs) of heavy metals : Importance for pollution induced community tolerance (PICT) determinations using the leucine incorporation method

Campillo-Cora, Claudia ; Soto-Gómez, Diego ; Arias-Estévez, Manuel ; Bååth, Erland LU and Fernández-Calviño, David LU (2021) In Soil Biology and Biochemistry 155.
Abstract

PICT (Pollution Induced Community Tolerance) to Cu is a useful and sensitive tool to assess the effects of Cu pollution in soils under laboratory conditions. However, in field situations, the absence of reference values, i.e. bacterial community tolerance to Cu baseline from non-polluted soils, make the method uncertain when we want to know if a soil is or is not polluted from a microbiological point of view. In order to shed some light on this topic, the PICT (Pollution Induced Community Tolerance) concept was used to determine the bacterial community tolerance to Cu in uncontaminated soils developed on five different parent materials, using log IC50 as a tolerance index. IC50 was calculated as the amount of Cu... (More)

PICT (Pollution Induced Community Tolerance) to Cu is a useful and sensitive tool to assess the effects of Cu pollution in soils under laboratory conditions. However, in field situations, the absence of reference values, i.e. bacterial community tolerance to Cu baseline from non-polluted soils, make the method uncertain when we want to know if a soil is or is not polluted from a microbiological point of view. In order to shed some light on this topic, the PICT (Pollution Induced Community Tolerance) concept was used to determine the bacterial community tolerance to Cu in uncontaminated soils developed on five different parent materials, using log IC50 as a tolerance index. IC50 was calculated as the amount of Cu that inhibit 50% of bacterial growth (estimated via the leucine incorporation method) in a bacterial suspension extracted from soil. With physico-chemical soil characteristics and type of parent material, a linear multiple regression equation was fitted explaining 80% of the variance in log IC50 values. This equation provides a useful tool to estimate the bacterial community tolerance to Cu baseline in a soil using the general soil characteristics, allowing for the more general use of PICT without the need of reference soils.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacterial community growth, Co-tolerance, PICT, Pollution, Risk assessment
in
Soil Biology and Biochemistry
volume
155
article number
108157
publisher
Elsevier
external identifiers
  • scopus:85100677951
ISSN
0038-0717
DOI
10.1016/j.soilbio.2021.108157
language
English
LU publication?
yes
id
3f702b84-6e0d-4d24-a1d1-6d435fbf8c80
date added to LUP
2021-02-22 09:51:30
date last changed
2021-02-22 09:51:30
@article{3f702b84-6e0d-4d24-a1d1-6d435fbf8c80,
  abstract     = {<p>PICT (Pollution Induced Community Tolerance) to Cu is a useful and sensitive tool to assess the effects of Cu pollution in soils under laboratory conditions. However, in field situations, the absence of reference values, i.e. bacterial community tolerance to Cu baseline from non-polluted soils, make the method uncertain when we want to know if a soil is or is not polluted from a microbiological point of view. In order to shed some light on this topic, the PICT (Pollution Induced Community Tolerance) concept was used to determine the bacterial community tolerance to Cu in uncontaminated soils developed on five different parent materials, using log IC<sub>50</sub> as a tolerance index. IC<sub>50</sub> was calculated as the amount of Cu that inhibit 50% of bacterial growth (estimated via the leucine incorporation method) in a bacterial suspension extracted from soil. With physico-chemical soil characteristics and type of parent material, a linear multiple regression equation was fitted explaining 80% of the variance in log IC<sub>50</sub> values. This equation provides a useful tool to estimate the bacterial community tolerance to Cu baseline in a soil using the general soil characteristics, allowing for the more general use of PICT without the need of reference soils.</p>},
  author       = {Campillo-Cora, Claudia and Soto-Gómez, Diego and Arias-Estévez, Manuel and Bååth, Erland and Fernández-Calviño, David},
  issn         = {0038-0717},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Soil Biology and Biochemistry},
  title        = {Bacterial community tolerance to Cu in soils with geochemical baseline concentrations (GBCs) of heavy metals : Importance for pollution induced community tolerance (PICT) determinations using the leucine incorporation method},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2021.108157},
  doi          = {10.1016/j.soilbio.2021.108157},
  volume       = {155},
  year         = {2021},
}