SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.
(2003) In Molecular Immunology 39(16). p.1035-1043- Abstract
- Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from... (More)
- Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from acidic domains located to the N-terminus and by recruiting the co-activator PIP to adjacent sites in a sequence specific manner. In contrast to PU.1 and PIP, SPI-C and PIP were unable to form a distinct ternary complex on the Ig λ light chain λ2–4 enhancer element, suggesting that SPI-C could act both as a positive and negative transcriptional regulator during B-lymphocyte differentiation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/114013
- author
- Carlsson, Robert LU ; Persson, Christine LU and Leanderson, Tomas LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Transcription, PU.1, His-tag, Modular, Substitution
- in
- Molecular Immunology
- volume
- 39
- issue
- 16
- pages
- 1035 - 1043
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- wos:000183119800006
- pmid:12749910
- scopus:0038056175
- ISSN
- 1872-9142
- DOI
- 10.1016/S0161-5890(03)00032-4
- language
- English
- LU publication?
- yes
- id
- 3f99614b-27cb-41bf-867f-12b77d4a7415 (old id 114013)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12749910&dopt=Abstract
- date added to LUP
- 2016-04-01 15:19:55
- date last changed
- 2022-04-14 21:41:24
@article{3f99614b-27cb-41bf-867f-12b77d4a7415,
abstract = {{Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from acidic domains located to the N-terminus and by recruiting the co-activator PIP to adjacent sites in a sequence specific manner. In contrast to PU.1 and PIP, SPI-C and PIP were unable to form a distinct ternary complex on the Ig λ light chain λ2–4 enhancer element, suggesting that SPI-C could act both as a positive and negative transcriptional regulator during B-lymphocyte differentiation.}},
author = {{Carlsson, Robert and Persson, Christine and Leanderson, Tomas}},
issn = {{1872-9142}},
keywords = {{Transcription; PU.1; His-tag; Modular; Substitution}},
language = {{eng}},
number = {{16}},
pages = {{1035--1043}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.}},
url = {{http://dx.doi.org/10.1016/S0161-5890(03)00032-4}},
doi = {{10.1016/S0161-5890(03)00032-4}},
volume = {{39}},
year = {{2003}},
}