Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems
(2017) In ChemPhysChem 18(10). p.1220-1223- Abstract
Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by... (More)
Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal-to-noise ratio data.
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- author
- Saldanha, Oliva ; Graceffa, Rita ; Hémonnot, Clément Y.J. ; Ranke, Christiane ; Brehm, Gerrit ; Liebi, Marianne LU ; Marmiroli, Benedetta ; Weinhausen, Britta ; Burghammer, Manfred and Köster, Sarah
- organization
- publishing date
- 2017-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cytoskeletal intermediate filaments, microfluidics, small-angle X-ray scattering, vimentin, water-in-oil emulsions
- in
- ChemPhysChem
- volume
- 18
- issue
- 10
- pages
- 4 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:28295928
- scopus:85017578770
- ISSN
- 1439-4235
- DOI
- 10.1002/cphc.201700221
- language
- English
- LU publication?
- yes
- id
- 3f9c216e-ddf5-4b4c-ac52-143516a0a7d9
- date added to LUP
- 2022-03-28 14:01:16
- date last changed
- 2024-07-02 01:15:37
@article{3f9c216e-ddf5-4b4c-ac52-143516a0a7d9, abstract = {{<p>Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal-to-noise ratio data.</p>}}, author = {{Saldanha, Oliva and Graceffa, Rita and Hémonnot, Clément Y.J. and Ranke, Christiane and Brehm, Gerrit and Liebi, Marianne and Marmiroli, Benedetta and Weinhausen, Britta and Burghammer, Manfred and Köster, Sarah}}, issn = {{1439-4235}}, keywords = {{cytoskeletal intermediate filaments; microfluidics; small-angle X-ray scattering; vimentin; water-in-oil emulsions}}, language = {{eng}}, number = {{10}}, pages = {{1220--1223}}, publisher = {{John Wiley & Sons Inc.}}, series = {{ChemPhysChem}}, title = {{Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems}}, url = {{http://dx.doi.org/10.1002/cphc.201700221}}, doi = {{10.1002/cphc.201700221}}, volume = {{18}}, year = {{2017}}, }