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Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems

Saldanha, Oliva ; Graceffa, Rita ; Hémonnot, Clément Y.J. ; Ranke, Christiane ; Brehm, Gerrit ; Liebi, Marianne LU ; Marmiroli, Benedetta ; Weinhausen, Britta ; Burghammer, Manfred and Köster, Sarah (2017) In ChemPhysChem 18(10). p.1220-1223
Abstract

Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by... (More)

Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal-to-noise ratio data.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytoskeletal intermediate filaments, microfluidics, small-angle X-ray scattering, vimentin, water-in-oil emulsions
in
ChemPhysChem
volume
18
issue
10
pages
4 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:28295928
  • scopus:85017578770
ISSN
1439-4235
DOI
10.1002/cphc.201700221
language
English
LU publication?
yes
id
3f9c216e-ddf5-4b4c-ac52-143516a0a7d9
date added to LUP
2022-03-28 14:01:16
date last changed
2024-03-11 09:24:45
@article{3f9c216e-ddf5-4b4c-ac52-143516a0a7d9,
  abstract     = {{<p>Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X-ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal-to-noise ratio data.</p>}},
  author       = {{Saldanha, Oliva and Graceffa, Rita and Hémonnot, Clément Y.J. and Ranke, Christiane and Brehm, Gerrit and Liebi, Marianne and Marmiroli, Benedetta and Weinhausen, Britta and Burghammer, Manfred and Köster, Sarah}},
  issn         = {{1439-4235}},
  keywords     = {{cytoskeletal intermediate filaments; microfluidics; small-angle X-ray scattering; vimentin; water-in-oil emulsions}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1220--1223}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{ChemPhysChem}},
  title        = {{Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems}},
  url          = {{http://dx.doi.org/10.1002/cphc.201700221}},
  doi          = {{10.1002/cphc.201700221}},
  volume       = {{18}},
  year         = {{2017}},
}