Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Localization of CGRP Receptor Components, CGRP, and Receptor Binding Sites in Human and Rhesus Cerebellar Cortex.

Eftekhari, Sajedeh LU ; Salvatore, Christopher A ; Gaspar, Renee C ; Roberts, Rhonda ; O'Malley, Stacey ; Zeng, Zhizhen and Edvinsson, Lars LU (2013) In Cerebellum 12(6). p.937-949
Abstract
The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high... (More)
The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high [(3)H]MK-3207 (CGRP receptor antagonist) binding densities in the molecular layer of rhesus cerebellar cortex; however, due to the limit of resolution of the autoradiographic image the exact cellular localization could not be determined. Similarly, [(125)I]CGRP binding was observed in the molecular layer and Purkinje cell layer of human cerebellum. CLR and RAMP1 mRNA was expressed within the Purkinje cell layer and some expression was found in the molecular layer. Immunofluorescence revealed expression of CGRP, CLR, and RAMP1 in the Purkinje cells and in cells in the molecular layer. Procalcitonin was found in the same localization. Recent research in the biology of cerebellum indicates that it may have a role in nociception. For the first time we have identified CGRP and CGRP receptor binding sites together with CGRP receptor expression through protein and mRNA localization in primate cerebellar cortex. These results point toward a functional role of CGRP in cerebellum. Further efforts are needed to evaluate this. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cerebellum
volume
12
issue
6
pages
937 - 949
publisher
Informa Healthcare
external identifiers
  • wos:000325827300020
  • pmid:23917876
  • scopus:84892444332
  • pmid:23917876
ISSN
1473-4230
DOI
10.1007/s12311-013-0509-4
language
English
LU publication?
yes
id
40064bdc-dbad-40e7-b420-a15c3535d5aa (old id 4006162)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23917876?dopt=Abstract
date added to LUP
2016-04-01 10:18:15
date last changed
2024-01-06 13:15:34
@article{40064bdc-dbad-40e7-b420-a15c3535d5aa,
  abstract     = {{The cerebellum is classically considered to be mainly involved in motor processing, but studies have suggested several other functions, including pain processing. Calcitonin-gene-related peptide (CGRP) is a neuropeptide involved in migraine pathology, where there is elevated release of CGRP during migraine attacks and CGRP receptor antagonists have antimigraine efficacy. In the present study, we examined CGRP and CGRP receptor binding sites and protein expression in primate cerebellar cortex. Additionally, mRNA expression of the CGRP receptor components, calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1), was examined. In addition, expression of procalcitonin was studied. We observed high [(3)H]MK-3207 (CGRP receptor antagonist) binding densities in the molecular layer of rhesus cerebellar cortex; however, due to the limit of resolution of the autoradiographic image the exact cellular localization could not be determined. Similarly, [(125)I]CGRP binding was observed in the molecular layer and Purkinje cell layer of human cerebellum. CLR and RAMP1 mRNA was expressed within the Purkinje cell layer and some expression was found in the molecular layer. Immunofluorescence revealed expression of CGRP, CLR, and RAMP1 in the Purkinje cells and in cells in the molecular layer. Procalcitonin was found in the same localization. Recent research in the biology of cerebellum indicates that it may have a role in nociception. For the first time we have identified CGRP and CGRP receptor binding sites together with CGRP receptor expression through protein and mRNA localization in primate cerebellar cortex. These results point toward a functional role of CGRP in cerebellum. Further efforts are needed to evaluate this.}},
  author       = {{Eftekhari, Sajedeh and Salvatore, Christopher A and Gaspar, Renee C and Roberts, Rhonda and O'Malley, Stacey and Zeng, Zhizhen and Edvinsson, Lars}},
  issn         = {{1473-4230}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{937--949}},
  publisher    = {{Informa Healthcare}},
  series       = {{Cerebellum}},
  title        = {{Localization of CGRP Receptor Components, CGRP, and Receptor Binding Sites in Human and Rhesus Cerebellar Cortex.}},
  url          = {{http://dx.doi.org/10.1007/s12311-013-0509-4}},
  doi          = {{10.1007/s12311-013-0509-4}},
  volume       = {{12}},
  year         = {{2013}},
}