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Molecular engineering of a thermostable carbohydrate-binding module

Cicortas Gunnarsson, Lavinia LU ; Nordberg Karlsson, Eva LU orcid ; Andersson, Mats ; Holst, Olle LU and Ohlin, Mats LU orcid (2006) Sixth Carbohydrate Bioengineering Meeting 24(1-2). p.31-37
Abstract
Structure-function studies are frequently practiced on the very diverse group of natural carbohydrate-binding modules in order to understand the target recognition of these proteins. We have taken a step further in the study of carbohydrate-binding modules and created variants with novel binding properties by molecular engineering of one such molecule of known 3D-structure. A combinatorial library was created from the sequence encoding a thermostable carbohydrate-binding module, CBM4-2 from a Rhodothermus marinus xylanase, and phage-display technology was successfully used for selection of variants with specificity towards different carbohydrate polymers (birchwood xylan, Avicel (TM), ivory nut mannan and recently also xyloglucan), as well... (More)
Structure-function studies are frequently practiced on the very diverse group of natural carbohydrate-binding modules in order to understand the target recognition of these proteins. We have taken a step further in the study of carbohydrate-binding modules and created variants with novel binding properties by molecular engineering of one such molecule of known 3D-structure. A combinatorial library was created from the sequence encoding a thermostable carbohydrate-binding module, CBM4-2 from a Rhodothermus marinus xylanase, and phage-display technology was successfully used for selection of variants with specificity towards different carbohydrate polymers (birchwood xylan, Avicel (TM), ivory nut mannan and recently also xyloglucan), as well as towards a glycoprotein (human IgG4). Our work not only generated a number of binders with properties that would suite a range of biotechnological applications, but analysis of selected binders also helped us to identify residues important for their specificities. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
carbohydrate-binding module, binding specificity, combinatorial, library, molecular engineering, phage-display, protein scaffold
host publication
Biocatalysis and Biotransformation
volume
24
issue
1-2
pages
31 - 37
publisher
Taylor & Francis
conference name
Sixth Carbohydrate Bioengineering Meeting
conference location
Barcelona, Spain
conference dates
2005-04-03 - 2005-04-06
external identifiers
  • wos:000239089700005
  • scopus:33646838036
ISSN
1029-2446
1024-2422
DOI
10.1080/10242420500518516
project
Designed carbohydrate binding modules and molecular probes
language
English
LU publication?
yes
id
6bd9f891-d050-40fd-b68f-05779f82ca8d (old id 401453)
date added to LUP
2016-04-01 12:02:02
date last changed
2021-02-17 08:17:55
@inproceedings{6bd9f891-d050-40fd-b68f-05779f82ca8d,
  abstract     = {Structure-function studies are frequently practiced on the very diverse group of natural carbohydrate-binding modules in order to understand the target recognition of these proteins. We have taken a step further in the study of carbohydrate-binding modules and created variants with novel binding properties by molecular engineering of one such molecule of known 3D-structure. A combinatorial library was created from the sequence encoding a thermostable carbohydrate-binding module, CBM4-2 from a Rhodothermus marinus xylanase, and phage-display technology was successfully used for selection of variants with specificity towards different carbohydrate polymers (birchwood xylan, Avicel (TM), ivory nut mannan and recently also xyloglucan), as well as towards a glycoprotein (human IgG4). Our work not only generated a number of binders with properties that would suite a range of biotechnological applications, but analysis of selected binders also helped us to identify residues important for their specificities.},
  author       = {Cicortas Gunnarsson, Lavinia and Nordberg Karlsson, Eva and Andersson, Mats and Holst, Olle and Ohlin, Mats},
  booktitle    = {Biocatalysis and Biotransformation},
  issn         = {1029-2446},
  language     = {eng},
  number       = {1-2},
  pages        = {31--37},
  publisher    = {Taylor & Francis},
  title        = {Molecular engineering of a thermostable carbohydrate-binding module},
  url          = {http://dx.doi.org/10.1080/10242420500518516},
  doi          = {10.1080/10242420500518516},
  volume       = {24},
  year         = {2006},
}