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U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner.

Ehrencrona, Hans LU ; Leuchowius, Karl-Johan; Hallböök, Helene; Nordgren, Ann; Thörn, Ingrid; Thorselius, Mia; Rosenquist, Richard; Söderberg, Ola and Sundström, Christer (2008) In European Journal of Haematology 81(3). p.218-225
Abstract
B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ... (More)
B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Oligonucleotide Array Sequence Analysis, Molecular Sequence Data, Male, Lymphoma, Leukocyte Count, B-Cell, Leukemia, Karyotyping, Fluorescence, In Situ Hybridization, Humans, Immunoglobulin, Genes, Flow Cytometry, Cytogenetic Analysis, Pair 18, Pair 14, Pair 12, Human, Chromosomes, Chromosome Aberrations, Cell Line, Bone Marrow Cells, Adult, Base Sequence, Phenotype, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins c-bcl-2, Proto-Oncogene Proteins c-myc, Translocation, Genetic
in
European Journal of Haematology
volume
81
issue
3
pages
218 - 225
publisher
Wiley-Blackwell
external identifiers
  • scopus:49349098502
ISSN
1600-0609
language
English
LU publication?
no
id
78cac79e-7fd3-41d3-b81c-3f5a9fe717d6 (old id 4021829)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18510704
http://dx.doi.org/10.1111/j.1600-0609.2008.01098.x
date added to LUP
2013-10-21 10:54:12
date last changed
2017-01-01 07:34:51
@article{78cac79e-7fd3-41d3-b81c-3f5a9fe717d6,
  abstract     = {B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1.},
  author       = {Ehrencrona, Hans and Leuchowius, Karl-Johan and Hallböök, Helene and Nordgren, Ann and Thörn, Ingrid and Thorselius, Mia and Rosenquist, Richard and Söderberg, Ola and Sundström, Christer},
  issn         = {1600-0609},
  keyword      = {Oligonucleotide Array Sequence Analysis,Molecular Sequence Data,Male,Lymphoma,Leukocyte Count,B-Cell,Leukemia,Karyotyping,Fluorescence,In Situ Hybridization,Humans,Immunoglobulin,Genes,Flow Cytometry,Cytogenetic Analysis,Pair 18,Pair 14,Pair 12,Human,Chromosomes,Chromosome Aberrations,Cell Line,Bone Marrow Cells,Adult,Base Sequence,Phenotype,Polymorphism,Single Nucleotide,Proto-Oncogene Proteins c-bcl-2,Proto-Oncogene Proteins c-myc,Translocation,Genetic},
  language     = {eng},
  number       = {3},
  pages        = {218--225},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Haematology},
  title        = {U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner.},
  volume       = {81},
  year         = {2008},
}