Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Emergence of carbapenem-resistant putative hypervirulent Klebsiella pneumoniae ST147 with a distinct hybrid plasmid in Türkiye

Vatansever, Cansel ; Akpınar, Anı ; Belge, Selin ; Kardan, Nihan ; Tanyolaç, Bade ; Kolsuz, Selin ; Boral, Jale LU orcid ; Ekinci, Güz ; Işler, Burcu and Ergonul, Onder , et al. (2026) In Emerging Microbes & Infections 15(1). p.1-13
Abstract
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) represents a growing clinical threat due to convergence of resistance and virulence determinants. We conducted a multicenter genomic epidemiology study analysing CR-hvKp isolates collected from 19 tertiary-care hospitals across Türkiye between 2018 and 2023. CR-hvKp isolates were subjected to whole-genome sequencing to investigate resistance and virulence determinants, plasmid replicon profiles, and phylogenetic relationships. The proportion of CR-hvKp among CR-Kp increased from 20.7% in 2018 to 33.1% by 2023. We identified ST2096 as the predominant clone in 2018–2019, largely associated with blaOXA-232. However, after 2022, ST147 emerged as the dominant CR-hvKp lineage,... (More)
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) represents a growing clinical threat due to convergence of resistance and virulence determinants. We conducted a multicenter genomic epidemiology study analysing CR-hvKp isolates collected from 19 tertiary-care hospitals across Türkiye between 2018 and 2023. CR-hvKp isolates were subjected to whole-genome sequencing to investigate resistance and virulence determinants, plasmid replicon profiles, and phylogenetic relationships. The proportion of CR-hvKp among CR-Kp increased from 20.7% in 2018 to 33.1% by 2023. We identified ST2096 as the predominant clone in 2018–2019, largely associated with blaOXA-232. However, after 2022, ST147 emerged as the dominant CR-hvKp lineage, characterized by co-carriage of blaNDM-5 and blaOXA-48, KL64 capsule, and exhibiting high antimicrobial resistance scores. Long-read sequencing revealed a 364-kb hybrid plasmid in an ST147 isolate, harbouring IncHI1B_pNDM and IncFIB_pNDM replicons. Notably, the ST147 hybrid plasmid (pPF302_hybrid) carried rmtF1 and arr-2, resistance genes that have not been previously reported in hybrid plasmids linked to ST147. These findings indicate ongoing acquisition of resistance determinants in ST147 and highlight its increased adaptive potential under antimicrobial pressure. Our results demonstrate a clear clonal shift in Türkiye’s CR-hvKp population from ST2096 to ST147, alongside evolving resistance patterns and the emergence of hybrid plasmids. These insights emphasise the need for ongoing genomic surveillance and robust molecular characterisation in regions facing high CR-hvKp prevalence. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Emerging Microbes & Infections
volume
15
issue
1
article number
2653280
pages
1 - 13
publisher
Nature Publishing Group
external identifiers
  • pmid:41914797
  • scopus:105035845144
ISSN
2222-1751
DOI
10.1080/22221751.2026.2653280
language
English
LU publication?
yes
id
403fc94e-c436-46e7-bbd9-d6cc004c0d1d
date added to LUP
2026-04-28 12:32:54
date last changed
2026-04-29 04:01:54
@article{403fc94e-c436-46e7-bbd9-d6cc004c0d1d,
  abstract     = {{Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) represents a growing clinical threat due to convergence of resistance and virulence determinants. We conducted a multicenter genomic epidemiology study analysing CR-hvKp isolates collected from 19 tertiary-care hospitals across Türkiye between 2018 and 2023. CR-hvKp isolates were subjected to whole-genome sequencing to investigate resistance and virulence determinants, plasmid replicon profiles, and phylogenetic relationships. The proportion of CR-hvKp among CR-Kp increased from 20.7% in 2018 to 33.1% by 2023. We identified ST2096 as the predominant clone in 2018–2019, largely associated with blaOXA-232. However, after 2022, ST147 emerged as the dominant CR-hvKp lineage, characterized by co-carriage of blaNDM-5 and blaOXA-48, KL64 capsule, and exhibiting high antimicrobial resistance scores. Long-read sequencing revealed a 364-kb hybrid plasmid in an ST147 isolate, harbouring IncHI1B_pNDM and IncFIB_pNDM replicons. Notably, the ST147 hybrid plasmid (pPF302_hybrid) carried rmtF1 and arr-2, resistance genes that have not been previously reported in hybrid plasmids linked to ST147. These findings indicate ongoing acquisition of resistance determinants in ST147 and highlight its increased adaptive potential under antimicrobial pressure. Our results demonstrate a clear clonal shift in Türkiye’s CR-hvKp population from ST2096 to ST147, alongside evolving resistance patterns and the emergence of hybrid plasmids. These insights emphasise the need for ongoing genomic surveillance and robust molecular characterisation in regions facing high CR-hvKp prevalence.}},
  author       = {{Vatansever, Cansel and Akpınar, Anı and Belge, Selin and Kardan, Nihan and Tanyolaç, Bade and Kolsuz, Selin and Boral, Jale and Ekinci, Güz and Işler, Burcu and Ergonul, Onder and Can, Fusun}},
  issn         = {{2222-1751}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{1}},
  pages        = {{1--13}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Emerging Microbes & Infections}},
  title        = {{Emergence of carbapenem-resistant putative hypervirulent Klebsiella pneumoniae ST147 with a distinct hybrid plasmid in Türkiye}},
  url          = {{http://dx.doi.org/10.1080/22221751.2026.2653280}},
  doi          = {{10.1080/22221751.2026.2653280}},
  volume       = {{15}},
  year         = {{2026}},
}