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Development and Application of Solid Phase Extraction Method for Polycyclic Aromatic Hydrocarbons in Water Samples in Johannesburg Area, South Africa

Sibiya, P.; Potgieter, M.; Cukrowska, E.; Jönsson, Jan Åke LU and Chimuka, L. (2012) In South African Journal of Chemistry 65. p.206-213
Abstract
A solid phase extraction (SPE) technique has been developed for the quantitative determination of polyaromatic hydrocarbons (PAHs) in aqueous samples. The SPE technique involved extraction of PAHs from a 100 mL sample containing 10 % methanol as a modifier onto C-18 cartridges. 40 % methanol in water was used as conditioning solvent, and 3 mL acetone:THF (1:1) as eluting solvent. After eluting, the extract was reduced to 1 mL under nitrogen and then analyzed by GC-MS. The extraction was optimized for the addition of organic modifier, sample load volume, conditioning solvent, washing solvent and eluting solvent. In order to evaluate the practical applicability of SPE technique, water samples were spiked with the PAHs to give final sample... (More)
A solid phase extraction (SPE) technique has been developed for the quantitative determination of polyaromatic hydrocarbons (PAHs) in aqueous samples. The SPE technique involved extraction of PAHs from a 100 mL sample containing 10 % methanol as a modifier onto C-18 cartridges. 40 % methanol in water was used as conditioning solvent, and 3 mL acetone:THF (1:1) as eluting solvent. After eluting, the extract was reduced to 1 mL under nitrogen and then analyzed by GC-MS. The extraction was optimized for the addition of organic modifier, sample load volume, conditioning solvent, washing solvent and eluting solvent. In order to evaluate the practical applicability of SPE technique, water samples were spiked with the PAHs to give final sample concentrations between 3 and 7 mu g L-1. Enrichment factors of 81-135 were achieved with relative standard deviations (RSDs) of less than 6 %. Recoveries obtained ranged from 81 to 135 %. Detection limits ranged from 20.0-52.0 ng L-1. The optimized method was validated by analyzing certified reference materials. The optimized method was then applied to spiked real river samples in and around the Johannesburg area, South Africa. The concentrations obtained varied from 22.0 to 1040.0 ng L-1. The RSDs were between 2.3 and 13 %. The overall order of PAHs levels was: phenanthrene > acenaphthene > naphthalene > fluoranthene > pyrene. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Solid phase extraction, polycyclic aromatic hydrocarbons, water samples, Johannesburg, South Africa
in
South African Journal of Chemistry
volume
65
pages
206 - 213
publisher
Bureau Scientific Publ
external identifiers
  • wos:000322108300024
  • scopus:84869477801
ISSN
0379-4350
language
English
LU publication?
yes
id
e27f8034-6f43-4090-a2ff-c52393d4e457 (old id 4042640)
date added to LUP
2013-10-07 16:40:46
date last changed
2017-10-01 04:02:49
@article{e27f8034-6f43-4090-a2ff-c52393d4e457,
  abstract     = {A solid phase extraction (SPE) technique has been developed for the quantitative determination of polyaromatic hydrocarbons (PAHs) in aqueous samples. The SPE technique involved extraction of PAHs from a 100 mL sample containing 10 % methanol as a modifier onto C-18 cartridges. 40 % methanol in water was used as conditioning solvent, and 3 mL acetone:THF (1:1) as eluting solvent. After eluting, the extract was reduced to 1 mL under nitrogen and then analyzed by GC-MS. The extraction was optimized for the addition of organic modifier, sample load volume, conditioning solvent, washing solvent and eluting solvent. In order to evaluate the practical applicability of SPE technique, water samples were spiked with the PAHs to give final sample concentrations between 3 and 7 mu g L-1. Enrichment factors of 81-135 were achieved with relative standard deviations (RSDs) of less than 6 %. Recoveries obtained ranged from 81 to 135 %. Detection limits ranged from 20.0-52.0 ng L-1. The optimized method was validated by analyzing certified reference materials. The optimized method was then applied to spiked real river samples in and around the Johannesburg area, South Africa. The concentrations obtained varied from 22.0 to 1040.0 ng L-1. The RSDs were between 2.3 and 13 %. The overall order of PAHs levels was: phenanthrene > acenaphthene > naphthalene > fluoranthene > pyrene.},
  author       = {Sibiya, P. and Potgieter, M. and Cukrowska, E. and Jönsson, Jan Åke and Chimuka, L.},
  issn         = {0379-4350},
  keyword      = {Solid phase extraction,polycyclic aromatic hydrocarbons,water samples,Johannesburg,South Africa},
  language     = {eng},
  pages        = {206--213},
  publisher    = {Bureau Scientific Publ},
  series       = {South African Journal of Chemistry},
  title        = {Development and Application of Solid Phase Extraction Method for Polycyclic Aromatic Hydrocarbons in Water Samples in Johannesburg Area, South Africa},
  volume       = {65},
  year         = {2012},
}